Rabbit NAPE-PLD Polyclonal Antibody | anti-NAPEPLD antibody

NAPE-PLD Antibody

Cat. Num. AAA9612330

• Gene Names: NAPEPLD; FMP30; C7orf18; NAPE-PLD
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Rabbit(88%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: NAPE-PLD; Polyclonal Antibody; NAPE-PLD Antibody; C7orf18; DKFZp781D1098; FMP30; Mbldc1; N acyl phosphatidylethanolamine hydrolyzing phospholipase D; N acyl phosphatidylethanolamine phospholipase D; N-acyl phosphatidylethanolamine phospholipase D; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; NAPE hydrolyzing phospholipase D; NAPE-hydrolyzing phospholipase D; NAPEP_HUMAN; NAPEPLD; anti-NAPEPLD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey; Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Rabbit(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NAPE-PLD Antibody detects endogenous levels of total NAPE-PLD.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-NAPEPLD antibody: ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ICC: 1:100-1:500; WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human NAPE-PLD, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from VERO cells, using NAPE-PLD Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain, using NAPE-PLD Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612330 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612330 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-NAPEPLD antibody

Function: Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid (PubMed:25684574, PubMed:14634025, PubMed:16527816). Responsible for the generation of these bioactive fatty acid ethanolamides (FAEs), including anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors (PubMed:14634025). As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose (By similarity).

Subcellular Location: Golgi apparatus membrane>Peripheral membrane protein. Early endosome membrane>Peripheral membrane protein. Nucleus envelope. Nucleus>Nucleoplasm. Note: Localized in the proximity of the cellular membranes likely through interaction with membrane phospholipids.

Tissue Specificity: Widely expressed. Highest expression in brain, kidney and testis (at protein level). Expressed in adipose tissue (at protein level).

Subunit Structure: Homodimer. Bile acids promote the assembly of inactive monomers into an active dimer and enable catalysis.

Similarity: Belongs to the NAPE-PLD family.

NCBI and Uniprot Product Information

• NCBI GI #: 170932481
• NCBI GeneID: 222236
• NCBI Accession #: NP_945341.3
• NCBI GenBank Nucleotide #: NP_945341.3
• UniProt Accession #: Q6IQ20
• Molecular Weight: Observed Molecular Weight: (Observed)45kD.; Predicted Molecular Weight: (Calculated)46kDa.
• NCBI Official Full Name: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
• NCBI Official Synonym Full Names: N-acyl phosphatidylethanolamine phospholipase D
• NCBI Official Symbol: NAPEPLD
• NCBI Official Synonym Symbols: FMP30; C7orf18; NAPE-PLD
• NCBI Protein Information: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
• UniProt Protein Name: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D
• Protein Family: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase
• UniProt Gene Name: NAPEPLD
• UniProt Synonym Gene Names: C7orf18; N-acyl phosphatidylethanolamine phospholipase D; NAPE-PLD; NAPE-hydrolyzing phospholipase D
• UniProt Entry Name: NAPEP_HUMAN

NCBI Description

NAPEPLD is a phospholipase D type enzyme that catalyzes the release of N-acylethanolamine (NAE) from N-acyl-phosphatidylethanolamine (NAPE) in the second step of the biosynthesis of N-acylethanolamine (Okamoto et al., 2004 [PubMed 14634025]).[supplied by OMIM, Oct 2008]

Uniprot Description

NAPEPLD: Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N- arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors. Belongs to the NAPE-PLD family.

Protein type: EC 3.1.4.54; Phospholipase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: cytoplasm

Molecular Function: zinc ion binding

Biological Process: phototransduction, visible light; phospholipid catabolic process; retinoid metabolic process