Rabbit SCAP Polyclonal Antibody | anti-SCAP antibody

SCAP Antibody

Cat. Num. AAA9612472

• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SCAP; Polyclonal Antibody; SCAP Antibody; KIAA0199; SCAP_HUMAN; SREBF chaperone; SREBF chaperone protein; SREBP cleavage activating protein; SREBP cleavage-activating protein; Sterol regulatory element binding protein cleavage-activating protein; Sterol regulatory element-binding protein cleavage-activating protein; anti-SCAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(88%), Bovine(100%), Horse(100%), Sheep(88%), Rabbit(88%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SCAP Antibody detects endogenous levels of total SCAP.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1279

• Applicable Applications for anti-SCAP antibody: ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ICC: 1:100-1:500; WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SCAP, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using SCAP Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from B16F10 cells, using SCAP Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612472 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612472 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-SCAP antibody

Function: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway (By similarity).

Post Translational Modifications: Ubiquitinated at Lys-454 and Lys-466. RNF145 triggers ubiquitination of SCAP, likely inhibiting SCAP:SREBPF2 complex transport to the Golgi apparatus and the subsequent processing/maturation of SREBPF2.

Subcellular Location: Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein. Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Subunit Structure: Membrane region forms a homotetramer. Forms a stable complex with SREBF1/SREBP1 or SREBF2/SREBP2 through its C-terminal cytoplasmic domain. Forms a ternary complex with INSIG1 or INSIG2 through its transmembrane domains at high sterol concentrations. Interacts with the SEC23/SEC24 complex in a SAR1-GTP-dependent manner through an ER export signal in its third cytoplasmic loop. Binds cholesterol through its SSC domain (By similarity). Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts with RNF139; the interaction inhibits the interaction of SCAP with SEC24B and hampering the ER to Golgi transport of the SCAP/SREBP complex.

Similarity: Cholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.Belongs to the WD repeat SCAP family.

NCBI and Uniprot Product Information

• NCBI GI #: 66932902
• NCBI GeneID: 22937
• NCBI Accession #: NP_036367.2
• NCBI GenBank Nucleotide #: NP_036367.2
• UniProt Accession #: Q12770
• Molecular Weight: Observed Molecular Weight: (Observed)139kD.; Predicted Molecular Weight: (Calculated)140kDa.
• NCBI Official Full Name: sterol regulatory element-binding protein cleavage-activating protein isoform 1
• NCBI Official Synonym Full Names: SREBF chaperone
• NCBI Official Symbol: SCAP
• NCBI Protein Information: sterol regulatory element-binding protein cleavage-activating protein
• UniProt Protein Name: Sterol regulatory element-binding protein cleavage-activating protein
• Protein Family: Sterol regulatory element-binding protein cleavage-activating protein
• UniProt Gene Name: SCAP
• UniProt Synonym Gene Names: KIAA0199; SCAP; SREBP cleavage-activating protein
• UniProt Entry Name: SCAP_HUMAN

NCBI Description

This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

SCAP: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Belongs to the WD repeat SCAP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; unfolded protein binding; cholesterol binding; protein complex binding

Biological Process: cholesterol metabolic process; regulation of fatty acid biosynthetic process; response to hypoxia; positive regulation of low-density lipoprotein receptor biosynthetic process; SREBP-mediated signaling pathway; negative regulation of cholesterol biosynthetic process; response to insulin stimulus; aging