Western Blot (WB)
(Western blot analysis of extracts from K562, using PGP Antibody. The lane on the left was treated with blocking peptide.)
Rabbit PGP Polyclonal Antibody | anti-PGP antibody
PGP Antibody
Gene Names
PGP; AUM; G3PP; PGPase
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PGP; Polyclonal Antibody; PGP Antibody; PGP_HUMAN; PGPase; Phosphoglycolate phosphatase; anti-PGP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(86%)
Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(86%)
Clonality
Polyclonal
Isotype
IgG
Specificity
PGP Antibody detects endogenous levels of total PGP.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
321
Applicable Applications for anti-PGP antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human PGP, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from K562, using PGP Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from K562, using PGP Antibody. The lane on the left was treated with blocking peptide.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612266 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612266) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612266 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612266) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Related Product Information for anti-PGP antibody
Function: Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed:26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).
Tissue Specificity: Detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The highest activities occur in skeletal muscle and cardiac muscle.
Subunit Structure: Homodimer.
Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
Tissue Specificity: Detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The highest activities occur in skeletal muscle and cardiac muscle.
Subunit Structure: Homodimer.
Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)34kD.
Predicted Molecular Weight: (Calculated)34kDa.
Predicted Molecular Weight: (Calculated)34kDa.
NCBI Official Full Name
glycerol-3-phosphate phosphatase
NCBI Official Synonym Full Names
phosphoglycolate phosphatase
NCBI Official Symbol
PGP
NCBI Official Synonym Symbols
AUM; G3PP; PGPase
NCBI Protein Information
glycerol-3-phosphate phosphatase
UniProt Protein Name
Phosphoglycolate phosphatase
Protein Family
UniProt Gene Name
PGP
UniProt Synonym Gene Names
PGP; PGPase
UniProt Entry Name
PGP_HUMAN
Uniprot Description
PGP: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.
Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.1.3.18; Phosphatase (non-protein); EC 3.1.3.48
Chromosomal Location of Human Ortholog: 16p13.3
Molecular Function: phosphoglycolate phosphatase activity; magnesium ion binding; protein tyrosine phosphatase activity
Biological Process: dephosphorylation; carbohydrate metabolic process
Research Articles on PGP
Similar Products
Product Notes
The PGP pgp (Catalog #AAA9612266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PGP Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(86%) and may cross-react with other species as described in the data sheet. AAA Biotech's PGP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the PGP pgp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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