Rabbit GOLPH3 Polyclonal Antibody | anti-GOLPH3 antibody

GOLPH3 Polyclonal Antibody

Cat. Num. AAA3012773

• Gene Names: GOLPH3; GOPP1; GPP34; MIDAS; Vps74
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: GOLPH3; Polyclonal Antibody; GOLPH3 Polyclonal Antibody; MIDAS; GPP34; Coat protein; Coat protein GPP34; FLJ90675; Golgi associated protein; Golgi peripheral membrane protein 1; 34 kDa; Golgi phosphoprotein 3 (coat protein); Golgi phosphoprotein 3; Golgi protein; GOLP3; Golph3; GOPP1; Mitochondrial DNA absence factor; anti-GOLPH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GOLPH3 polyclonal antibody detects endogenous levels of GOLPH3 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 298

• Applicable Applications for anti-GOLPH3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Immunocytochemistry (ICC)

(ICC staining GOLPH3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining GOLPH3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-GOLPH3 antibody

GOLPH3 (golgi phosphoprotein 3), also known as GOPP1, GPP34 or MIDAS, is a 298 amino acid protein that localizes to both the cytoplasm and the Golgi stack membrane where it is thought to play a regulatory role in protein trafficking within the Golgi. GOLPH3 is subject to post-translational phosphorylation and is encoded by a gene which maps to human chromosome 5. Chromosome 5 contains 181 million base pairs and comprises nearly 6% of the human genome. Chromosome 5 is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene. Treacher Collins syndrome is also chromosome 5-associated and is caused by insertions or deletions within the TCOF1 gene. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome.

NCBI and Uniprot Product Information

• NCBI GI #: 15082415
• NCBI GeneID: 64083
• UniProt Accession #: Q9H4A6; Q9CRA5; Q9ERE4
• Molecular Weight: 34kDa
• NCBI Official Full Name: Golgi phosphoprotein 3 (coat-protein)
• NCBI Official Synonym Full Names: golgi phosphoprotein 3
• NCBI Official Symbol: GOLPH3
• NCBI Official Synonym Symbols: GOPP1; GPP34; MIDAS; Vps74
• NCBI Protein Information: Golgi phosphoprotein 3
• UniProt Protein Name: Golgi phosphoprotein 3
• Protein Family: Golgi phosphoprotein
• UniProt Gene Name: GOLPH3
• UniProt Synonym Gene Names: GPP34; MIDAS
• UniProt Entry Name: GOLP3_HUMAN

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

GOLPH3: Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. Belongs to the GOLPH3/VPS74 family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: nucleoplasm; Golgi apparatus; Golgi cisterna; mitochondrion; plasma membrane; mitochondrial intermembrane space; trans-Golgi network; cytosol; endosome

Molecular Function: protein binding; enzyme binding

Biological Process: lamellipodium biogenesis; cell proliferation; cell migration; positive regulation of protein secretion; glycoprotein biosynthetic process; Golgi to plasma membrane protein transport; protein secretion; gene expression; Golgi vesicle budding; leukocyte tethering or rolling; protein retention in Golgi; Golgi organization and biogenesis; positive regulation of TOR signaling pathway