Chromodomain Helicase DNA Binding Protein 3 (CHD3) Recombinant Protein | CHD3 recombinant protein

Recombinant Chromodomain Helicase DNA Binding Protein 3 (CHD3)

Cat. Num. AAA2018219

• Gene Names: Chd3; Chd7; Prp7; Prp9-1; AF020312; 2600010P09Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Chromodomain Helicase DNA Binding Protein 3 (CHD3); Recombinant Chromodomain Helicase DNA Binding Protein 3 (CHD3); CHD3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SEKGDGVR TPLDKDDTEN QEEKPEKNSK VGEKMEAEVD SPSPAPSLGE RLEHRKILLE DEAPGVPGET EPEPGYRGDR EKSASEPTPG ERGEEKPLDV QEHRERTEGE TGDLGKREDV KADRELRLGP PRDEPRPNGR REEKVEKPRF MFNIADGGFT ELHTLWQNEE RAAISSGKLN EIWHRRHDYWLLAGIVLHGY ARWQDIQNDA
• Sequence Length: 2055

• Applicable Applications for CHD3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser1613~Ala1820 (Accession # B1AR17) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 5.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 357527416
• NCBI GeneID: 216848
• NCBI Accession #: NP_666131.3
• NCBI GenBank Nucleotide #: NM_146019.4
• UniProt Accession #: B1AR17
• Molecular Weight: 27.5kDa
• NCBI Official Full Name: chromodomain helicase DNA binding protein 3
• NCBI Official Synonym Full Names: chromodomain helicase DNA binding protein 3
• NCBI Official Symbol: Chd3
• NCBI Official Synonym Symbols: Chd7; Prp7; Prp9-1; AF020312; 2600010P09Rik
• NCBI Protein Information: chromodomain helicase DNA binding protein 3; Mi-2 alpha
• UniProt Protein Name: Protein Chd3
• Protein Family: Chromodomain-helicase-DNA-binding protein
• UniProt Gene Name: Chd3
• UniProt Entry Name: B1AR17_MOUSE

Uniprot Description

CHD-3: Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity. Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts (via its C-terminal) with HABP4. Interacts with PCNT; the interaction regulates centrosome integrity. Widely expressed. Belongs to the SNF2/RAD54 helicase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.12; Helicase; DNA-binding

Cellular Component: centrosome; intermediate filament cytoskeleton; cytoplasm; nucleolus; NuRD complex; nucleus

Molecular Function: protein binding

Biological Process: chromatin assembly or disassembly; centrosome organization and biogenesis; spindle organization and biogenesis