Rat anti-Mouse CD8a Monoclonal Antibody | anti-CD8a antibody

Anti-Ms CD8a Alexa Fluor488

• Gene Names: Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD8a; Monoclonal Antibody; Anti-Ms CD8a Alexa Fluor488; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331; CD8a molecule; anti-CD8a antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone Number: 53-6.7
• Specificity: The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34kDa; alpha chain of the CD8 antigen).
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CD8a antibody: Flow Cytometry (FC/FACS) (QC tested), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: Recommended dilution: 2ug/ml
• Conjugation: Alexa Fluor488
• Immunogen: Murine spleen cells
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) Alexa Fluor 488 antibody (concentration in sample 1 ug/ml).)

Flow Cytometry (FC/FACS)

(Separation of murine CD8a positive splenocytes (red-filled) from CD8a negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) Alexa Fluor 488 antibody (concentration in sample 1 ug/ml).)

Related Product Information for anti-CD8a antibody

The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector Functional Application (FUNC)tion. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the Functional Application (FUNC)tional diversity of the TCR signaling.

References

*Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18263815ordin,*Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5102-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/16549792ordin,*Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb. J Immunol. 2006 Jul 1;177(1):306-14., URL: https://www.ncbi.nlm.nih.gov/pubmed/16785526ordin,*Kosy, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1;175(5):3309-17., URL: https://www.ncbi.nlm.nih.gov/pubmed/16116223ordin,*Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan;109(2):183-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/11805130ordin,*Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest. 2004 Aug;114(4):582-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15314695ordin,*Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5557-61., URL: https://www.ncbi.nlm.nih.gov/pubmed/1608966ordina,*Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/6156984ordina,*Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/398327ordinal

NCBI and Uniprot Product Information

• NCBI GI #: 124487437
• NCBI GeneID: 12525
• NCBI Accession #: NP_001074579.1
• NCBI GenBank Nucleotide #: NM_001081110.2
• UniProt Accession #: P01731
• Molecular Weight: 27456 MW
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8 antigen, alpha chain
• NCBI Official Symbol: Cd8a
• NCBI Official Synonym Symbols: Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: Cd8a
• UniProt Synonym Gene Names: Lyt-2; Lyt2

Uniprot Description

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.