Rabbit anti-Human SEPT12 Polyclonal Antibody | anti-SEPT12 antibody

SEPT12, ID (SEPT12, Septin-12)

Cat. Num. AAA643801

• Gene Names: SEPT12; SPGF10
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SEPT12; Polyclonal Antibody; ID (SEPT12; Septin-12); Anti -SEPT12; anti-SEPT12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SEPT12 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: SEPT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-244 amino acids from the Central region of human SEPT12.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SEPT12 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SEPT12 antibody detected the SEPT12 protein (arrow).)

Related Product Information for anti-SEPT12 antibody

Septins, such as SEPT12, are conserved GTP-binding proteins that function as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeleton remodeling, as well as infection, neurodegeneration, and neoplasia (Hall et al., 2005 [PubMed 15915442]).

Product Categories/Family for anti-SEPT12 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 226492499
• NCBI GeneID: 124404
• NCBI Accession #: NP_001147930.1
• NCBI GenBank Nucleotide #: NM_001154458.2
• UniProt Accession #: Q8IYM1; Q0P6B0; Q1PBH0; Q96LL0
• Molecular Weight: 40,748 Da
• NCBI Official Full Name: septin-12 isoform 1
• NCBI Official Synonym Full Names: septin 12
• NCBI Official Symbol: SEPT12
• NCBI Official Synonym Symbols: SPGF10
• NCBI Protein Information: septin-12
• UniProt Protein Name: Septin-12
• UniProt Gene Name: SEPT12
• UniProt Entry Name: SEP12_HUMAN

NCBI Description

This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SEPT12: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). Defects in SEPT12 are the cause of spermatogenic failure 10 (SPGF10). An infertility disorder caused by spermatogenesis defects. It results in decreased sperm motility, concentration, and multiple sperm structural defects. The most prominent feature is a defective sperm annulus, a ring structure that demarcates the midpiece and the principal piece of the sperm tail. Belongs to the septin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Hydrolase; Cytoskeletal

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: microtubule cytoskeleton; perinuclear region of cytoplasm; stress fiber; spindle; midbody; cleavage furrow

Molecular Function: protein binding; GDP binding; protein homodimerization activity; GTP binding; phosphoinositide binding

Biological Process: cell division; cell cycle

Disease: Spermatogenic Failure 10