Rabbit AKR1C2 Polyclonal Antibody | anti-AKR1C2 antibody

AKR1C2 Polyclonal Antibody

Cat. Num. AAA2564604

• Gene Names: AKR1C2; DD; DD2; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity purification
• Synonyms: AKR1C2; Polyclonal Antibody; AKR1C2 Polyclonal Antibody; AKR1C-pseudo; BABP; DD; DD-2; DD/BABP; DD2; DDH2; HAKRD; HBAB; MCDR2; SRXY8; TDD; anti-AKR1C2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-AKR1C2 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:100
• Immunogen: Recombinant fusion protein of human AKR1C2 (NP_9953171)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines using AKR1C2 Polyclonal Antibody at dilution of 1:1000.)

Immunofluorescence (IF)

(Immunofluorescence analysis of L929 cells using AKR1C2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U-2 OS cells using AKR1C2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Related Product Information for anti-AKR1C2 antibody

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-AKR1C2 antibody

Cancer; Metabolism; Signal Transduction; Polyclonal Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 207028673
• NCBI GeneID: 1646
• NCBI Accession #: NP_001128713.1
• NCBI GenBank Nucleotide #: NM_001135241.2
• UniProt Accession #: P52895; Q14133; Q5SR16; Q7M4N1; Q96A71; A8K2N9; B4DKR9
• Molecular Weight: 36,735 Da
• NCBI Official Full Name: aldo-keto reductase family 1 member C2 isoform 2
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member C2
• NCBI Official Symbol: AKR1C2
• NCBI Official Synonym Symbols: DD; DD2; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo
• NCBI Protein Information: aldo-keto reductase family 1 member C2; DD-2; DD/BABP; 3-alpha-HSD3; pseudo-chlordecone reductase; type II dihydrodiol dehydrogenase; chlordecone reductase homolog HAKRD; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; dihydrodiol dehydrogenase 2; bile a
• UniProt Protein Name: Aldo-keto reductase family 1 member C2
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C2
• UniProt Synonym Gene Names: DDH2; DD-2; DD2; DD/BABP
• UniProt Entry Name: AK1C2_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.1.1.357; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.3.1.20

Chromosomal Location of Human Ortholog: 10p15-p14

Cellular Component: cytoplasm

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; carboxylic acid binding; bile acid binding; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity

Biological Process: steroid metabolic process; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; epithelial cell differentiation; digestion; positive regulation of cell proliferation; progesterone metabolic process; prostaglandin metabolic process

Disease: 46,xy Sex Reversal 8