Rabbit GOLPH3 Polyclonal Antibody | anti-GOLPH3 antibody

GOLPH3 Antibody

Cat. Num. AAA9402480

• Gene Names: GOLPH3; GOPP1; GPP34; MIDAS; Vps74
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: GOLPH3; Polyclonal Antibody; GOLPH3 Antibody; Golgi phosphoprotein 3; GOPP1; GPP34; MIDAS; anti-GOLPH3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 298

• Applicable Applications for anti-GOLPH3 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 14 amino acid peptide from near the amino terminus of human GOLPH3.
• Target Name: GOLPH3
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of GOLPH3 in rat lung tissue lysate with GOLPH3 antibody at (A) 0.5 and (B) 1 ug/mL.)

Related Product Information for anti-GOLPH3 antibody

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Product Categories/Family for anti-GOLPH3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 15082415
• NCBI GeneID: 64083
• Molecular Weight: 33,811 Da
• NCBI Official Full Name: Golgi phosphoprotein 3 (coat-protein)
• NCBI Official Synonym Full Names: golgi phosphoprotein 3 (coat-protein)
• NCBI Official Symbol: GOLPH3
• NCBI Official Synonym Symbols: GOPP1; GPP34; MIDAS; Vps74
• NCBI Protein Information: Golgi phosphoprotein 3
• UniProt Protein Name: Golgi phosphoprotein 3
• Protein Family: Golgi phosphoprotein
• UniProt Gene Name: GOLPH3
• UniProt Synonym Gene Names: GPP34; MIDAS
• UniProt Entry Name: GOLP3_HUMAN

NCBI Description

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

GOLPH3: Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. Belongs to the GOLPH3/VPS74 family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: nucleoplasm; Golgi apparatus; Golgi cisterna; mitochondrion; plasma membrane; mitochondrial intermembrane space; trans-Golgi network; cytosol; endosome

Molecular Function: protein binding; enzyme binding

Biological Process: lamellipodium biogenesis; cell proliferation; cell migration; glycoprotein biosynthetic process; positive regulation of protein secretion; Golgi to plasma membrane protein transport; protein secretion; gene expression; Golgi vesicle budding; leukocyte tethering or rolling; protein retention in Golgi; Golgi organization and biogenesis; positive regulation of TOR signaling pathway