Western Blot (WB)
(Western Blot Test: Western blot detection of endogenous Gai3 protein in normal rat kidney (NRK) cell line. MBS613101 dilution: 1:1000; Goat anti-IgY Fc-HRP: 1:10,000)
Chicken Gai3 Polyclonal Antibody
Gai3 (GNAI3, Guanine Nucleotide Binding Protein (G Protein) Alpha Inhibiting Activity Polypeptide 3, 87U6, FLJ26559, G(i) alpha-3, Guanine nucleotide-binding protein G(k) subunit alpha)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Gai3; Polyclonal Antibody; Gai3 (GNAI3; Guanine Nucleotide Binding Protein (G Protein) Alpha Inhibiting Activity Polypeptide 3; 87U6; FLJ26559; G(i) alpha-3; Guanine nucleotide-binding protein G(k) subunit alpha); Anti -Gai3 (GNAI3; anti-Gai3 antibody
Host
Chicken
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes human GNAI3. Species Crossreactivity: Mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Applicable Applications for anti-Gai3 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunocytochemistry and Western Blot.
Dilution: Immunocytochemistry: 1:200
Western Blot: 1:500
Dilution: Immunocytochemistry: 1:200
Western Blot: 1:500
Immunogen
C-terminal peptide (KNNLKECGLY)
Positive Control
AtT 20 and Normal rat kidney (NRK) cell line.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot Test: Western blot detection of endogenous Gai3 protein in normal rat kidney (NRK) cell line. MBS613101 dilution: 1:1000; Goat anti-IgY Fc-HRP: 1:10,000)
Western Blot (WB)
(Western Blot Test: Western blot detection of endogenous Gai3 protein in normal rat kidney (NRK) cell line. MBS613101 dilution: 1:1000; Goat anti-IgY Fc-HRP: 1:10,000)
Immunocytochemistry (ICC)
(Immunocytochemistry Staining: Immunocytochemistry staining of endogenous Gai3 protein in AtT-20 and normal rat kidney (NRK) cell lines. MBS613101 dilution: 1:200; Mouse anti-IgY Fc- Alexa 594: 1:200)
Immunocytochemistry (ICC)
(Immunocytochemistry Staining: Immunocytochemistry staining of endogenous Gai3 protein in AtT-20 and normal rat kidney (NRK) cell lines. MBS613101 dilution: 1:200; Mouse anti-IgY Fc- Alexa 594: 1:200)
Related Product Information for anti-Gai3 antibody
Gai3 is the a subunit of inhibitory trimeric G protein (Gi), which inhibits adenylate cyclase following its G-protein coupled receptors (GPCR) such as a2-adrenergic receptors are activated. Gai3 can be inactivated by pertussis toxin.
Product Categories/Family for anti-Gai3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
GAI/RGA-like protein
Similar Products
Product Notes
The Gai3 (Catalog #AAA613101) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Gai3 (GNAI3, Guanine Nucleotide Binding Protein (G Protein) Alpha Inhibiting Activity Polypeptide 3, 87U6, FLJ26559, G(i) alpha-3, Guanine nucleotide-binding protein G(k) subunit alpha) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Gai3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Immunocytochemistry and Western Blot. Dilution: Immunocytochemistry: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the Gai3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Gai3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
