Immunohistochemistry (IHC)
(Representative images from a previous lot. Optimal staining of MBS620758 with Citrate pH 6.0 epitope retrieval: rat cerebellum Representative staining pattern/morphology in rat cerebellum using MBS620758. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of MBS620758 was diluted to 1:100, using IHC-Select® Detection with HRP-DAB)
Rabbit G Protein Gi alpha1/alpha2 Polyclonal Antibody
G Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2)
Reactivity
Bovine, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
PurifiedPurified
Synonyms
G Protein Gi alpha1/alpha2; Polyclonal Antibody; G Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein); alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein); alpha inhibiting activity polypeptide 2); Anti -G Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein); anti-G Protein Gi alpha1/alpha2 antibody
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human G protein subunit alphai1 and alpha2. Species Crossreactivity: mouse, rat, bovine.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.08% sodium azide.
Applicable Applications for anti-G Protein Gi alpha1/alpha2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:100
Dilution: Western Blot: 1:500-1:1000
Immunohistochemistry (paraffin): 1:100
Immunogen
Synthetic peptide corresponding to aa345-354 of Gi alpha1 and 346-355 of Gi alpha2.
Positive Control
L6 Cell Lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Representative images from a previous lot. Optimal staining of MBS620758 with Citrate pH 6.0 epitope retrieval: rat cerebellum Representative staining pattern/morphology in rat cerebellum using MBS620758. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of MBS620758 was diluted to 1:100, using IHC-Select® Detection with HRP-DAB)
Immunohistochemistry (IHC)
(Representative images from a previous lot. Optimal staining of MBS620758 with Citrate pH 6.0 epitope retrieval: rat cerebellum Representative staining pattern/morphology in rat cerebellum using MBS620758. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of MBS620758 was diluted to 1:100, using IHC-Select® Detection with HRP-DAB)
Chemiluminescence
(L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620758 (1:500). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates MBS620758(~40kD).)
Chemiluminescence
(L6 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620758 (1:500). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates MBS620758(~40kD).)
Testing Data
(Representative image from a previous lot. Huvec lysate (Lane 1), A431 lysate (Lane 2), L6 lysate (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620758 (1:500) Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates G protein Gialpha 1 and 2 (~40kD).)
Testing Data
(Representative image from a previous lot. Huvec lysate (Lane 1), A431 lysate (Lane 2), L6 lysate (Lane 3) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS620758 (1:500) Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates G protein Gialpha 1 and 2 (~40kD).)
Related Product Information for anti-G Protein Gi alpha1/alpha2 antibody
GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).
Product Categories/Family for anti-G Protein Gi alpha1/alpha2 antibody
Similar Products
Product Notes
The G Protein Gi alpha1/alpha2 (Catalog #AAA620758) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The G Protein Gi alpha1/alpha2 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 / guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2) reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's G Protein Gi alpha1/alpha2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:100. Researchers should empirically determine the suitability of the G Protein Gi alpha1/alpha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G Protein Gi alpha1/alpha2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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