Chicken anti-Human, Mouse Coronin 1A Polyclonal Antibody | anti-CORO1A antibody

Coronin 1A

Cat. Num. AAA615177

• Gene Names: CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA; FLJ41407; MGC117380
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Coronin 1A; Polyclonal Antibody; Anti -Coronin 1A; anti-CORO1A antibody

• Host: Chicken
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human and mouse Coronin 1A.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-CORO1A antibody: Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunohistochemistry and Immunocytochemistry.; Dilution: Immunohistochemistry: 1:1000; Immunocytochemistry: 1:1000
• Immunogen: Peptide corresponded to a region of the Coronin 1A gene sequence shared between the mouse (NM_009898) and human (NM_007074) gene products.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(MBS615177 staining of cultured adult mouse brain cells.)

Related Product Information for anti-CORO1A antibody

Coronin 1A is a protein that in humans is encoded by the CORO1A gene. It has been implicated in both T-cell mediated immunity and mitochondrial apoptosis. In a recent genome-wide longevity study, its expression levels were found to be negatively associated both with age at the time of blood sample and the survival time after blood draw.

Human Coronin 1A is a 51,026 dalton protein (461 amino acids) expressed intrcellularly in macrophages and other cell types of the immune system. In the CNS, coronin 1A is expressed exclusively by microglial cells. Although its function is not entirely clear, there are reasons to believe that coronin serves as a linkage protein between the actin cytoskeleton and the plasma membrane, and perhaps the extracellular matrix.
Coronin was originally discovered in Dictyostelium, where it was found to be involved in the chemotactic response of these ameboid cells. The name derives from the fact that the protein is localized at the leading edge or crown of these highly motile cells. The name derives from Corona, which is latin for crown. Coronin homologues have been found in yeast, C. elegans, Drosophila and many other species, and a family them are known in mammals. All coronins belong to the WD40 or WD family of proteins, the prototype or which is the beta subunit of trimeric G-proteins. The beta subunit proteins all have a beautiful conserved wheel-like seven bladed beta-propeller structure, with each blade being formed by four beta strands generated by one of the WD sequence repeats. The G protein beta subunits are believed to function as general purpose binding adapters, mediating numerous regulatory binding interactions between G proteins, G protein coupled receptors and G protein effectors. Although sequence analyis suggested that coronin 1A only possess 5 of the these WD repeats, recent structural studies show that, like the G protein beta-subunits, there are seven beta-propellers, generating a compact 7 bladed propellor.
Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis. In mammals there are at least five major coronin proteins, named coronins 1 to 5 in one nomenclature. Another nomenclature divides these five proteins in coronins 1A and 1B, 2A, 2B and 2C (see the HUGO (Human Genone Organization) Gene Nomenclature Committee link for this family). The various coronin proteins have several other alternate names, since they were discovered independently by several different groups. The mammalian coronin family members are abundant components of eukaryotic cells, and each type has a restricted cell type specific expression pattern. Coronin 1A is the mammalian coronin most similar in protein sequence to the Dictyostelium protein and is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils. Coronin 1a is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils. Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type. Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors. Since coronin 1A is a constitutive component of microglia, the coronin 1A antibody can be used to study both quiescent and activated microglia.

Product Categories/Family for anti-CORO1A antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5902134
• NCBI GeneID: 11151
• NCBI Accession #: NP_009005.1
• NCBI GenBank Nucleotide #: NM_007074.3
• UniProt Accession #: P31146; Q2YD73; B2RBL1; Q59G88; Q6ZW98
• Molecular Weight: 51,026 Da
• NCBI Official Full Name: coronin-1A
• NCBI Official Synonym Full Names: coronin, actin binding protein, 1A
• NCBI Official Symbol: CORO1A
• NCBI Official Synonym Symbols: p57; TACO; CLABP; HCORO1; CLIPINA; FLJ41407; MGC117380
• NCBI Protein Information: coronin-1A; clipin-A; OTTHUMP00000163017; coronin-like protein A; coronin-like protein p57; tryptophan aspartate-containing coat protein
• UniProt Protein Name: Coronin-1A
• Protein Family: Coronin
• UniProt Gene Name: CORO1A
• UniProt Synonym Gene Names: CORO1
• UniProt Entry Name: COR1A_HUMAN

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq]

Uniprot Description

coronin 1A: May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria- infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Belongs to the WD repeat coronin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cortical actin cytoskeleton; protein complex; early endosome; immunological synapse; actin filament; phagocytic vesicle; intercellular junction; phagocytic vesicle membrane; membrane; lamellipodium; axon; cytoplasm; plasma membrane; nucleus; phagocytic cup

Molecular Function: protein C-terminus binding; actin filament binding; protein binding; protein homodimerization activity; cytoskeletal protein binding; phosphoinositide 3-kinase binding

Biological Process: negative regulation of vesicle fusion; leukocyte chemotaxis; actin filament organization; cell-substrate adhesion; regulation of release of sequestered calcium ion into cytosol; phagocytosis; uropod organization and biogenesis; phagolysosome formation; regulation of cell shape; regulation of actin filament polymerization; positive chemotaxis; homeostasis of number of cells within a tissue; calcium ion transport; negative regulation of actin nucleation; innate immune response; T cell homeostasis; positive regulation of T cell proliferation; negative regulation of neuron apoptosis; cell motility; actin cytoskeleton organization and biogenesis; positive regulation of cell migration

Disease: Immunodeficiency 8