Rabbit anti-Human GNAS Polyclonal Antibody | anti-GNAS antibody

GNAS, CT (GNAS)

Cat. Num. AAA6013420

• Gene Names: GNAS; GNAS1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: GNAS; Polyclonal Antibody; CT (GNAS); Anti -GNAS; anti-GNAS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-GNAS antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Immunofluorescence: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: GNAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human GNAS.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of GNAS (arrow) of 293 cell lysates (2ug/lane) using MBS6013420 using either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene.)

Western Blot (WB)

(Western Blot analysis in 293,NCI-H292 cell line lysates (35ug/lane) using MBS6013420. This demonstrates that MBS6013420 detected the GNAS protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6013420 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of 293 cell using MBS6013420 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS6013420(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GNAS antibody

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.

Product Categories/Family for anti-GNAS antibody

Antibodies; Abs to Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 30794332
• NCBI GeneID: 281793
• NCBI Accession #: NP_851364.1
• NCBI GenBank Nucleotide #: NM_181021.3
• UniProt Accession #: P04896; Q0II85; Q3SZE7; O18979
• Molecular Weight: 45,709 Da
• NCBI Official Full Name: GNAS complex locus
• NCBI Official Synonym Full Names: GNAS complex locus<
• NCBI Official Symbol: GNAS
• NCBI Official Synonym Symbols: GNAS1
• NCBI Protein Information: GNAS complex locus; NESP55; neuroendocrine secretory protein 55; adenylate cyclase-stimulating G alpha protein; guanine nucleotide-binding protein G(s) subunit alpha isoforms; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1
• UniProt Protein Name: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
• Protein Family: Protein
• UniProt Gene Name: GNAS
• UniProt Synonym Gene Names: GNAS1
• UniProt Entry Name: GNAS2_BOVIN

Uniprot Description

Function: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(s) protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Stimulates the Ras signaling pathway via RAPGEF2

By similarity.

Subunit structure: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Subcellular location: Cell membrane; Lipid-anchor

By similarity.

Miscellaneous: The GNAS locus is imprinted in a complex manner, giving rise to distinct paternally, maternally and biallelically expressed proteins.

Sequence similarities: Belongs to the G-alpha family. G(s) subfamily.