Goat anti-Human FTCD Polyclonal Antibody | anti-FTCD antibody

FTCD, NT (Formimidoyltransferase-cyclodeaminase, Formiminotransferase-cyclodeaminase, LCHC1)

Cat. Num. AAA645700

• Gene Names: FTCD; LCHC1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: FTCD; Polyclonal Antibody; NT (Formimidoyltransferase-cyclodeaminase; Formiminotransferase-cyclodeaminase; LCHC1); Anti -FTCD; anti-FTCD antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human FTCD. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-FTCD antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:64,000; Western Blot: 0.03-0.5ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3.75ug/ml
• Immunogen: Synthetic peptide corresponding to SQLVECVPNFSEGKNQ from the N-terminal of FTCD
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Liver (formalin-fixed, paraffin-embedded) stained with FTCD antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Chemiluminescence

((0.03ug/ml) Staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-FTCD antibody

FTCD is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool.

Product Categories/Family for anti-FTCD antibody

Antibodies; Abs to Golgi Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 11140815
• NCBI GeneID: 10841
• NCBI Accession #: NP_006648.1
• NCBI GenBank Nucleotide #: NM_006657.2
• UniProt Accession #: O95954; Q86V03; Q9HCT4; Q9HCT5; Q9HCT6; Q9UHJ2; B9EGD0
• Molecular Weight: 58,927 Da
• NCBI Official Full Name: formimidoyltransferase-cyclodeaminase
• NCBI Official Synonym Full Names: formimidoyltransferase cyclodeaminase
• NCBI Official Symbol: FTCD
• NCBI Official Synonym Symbols: LCHC1
• NCBI Protein Information: formimidoyltransferase-cyclodeaminase; formiminotransferase cyclodeaminase; formiminotransferase-cyclodeaminase
• UniProt Protein Name: Formimidoyltransferase-cyclodeaminase
• Protein Family: Formimidoyltransferase-cyclodeaminase
• UniProt Gene Name: FTCD
• UniProt Synonym Gene Names: FTCD
• UniProt Entry Name: FTCD_HUMAN

NCBI Description

The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]

Uniprot Description

Function: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.Binds and promotes bundling of vimentin filaments originating from the Golgi

By similarity.

Catalytic activity: 5-formimidoyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formimidoyl-L-glutamate.5-formyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formyl-L-glutamate.5-formimidoyltetrahydrofolate = 5,10-methenyltetrahydrofolate + NH3.

Cofactor: Pyridoxal phosphate.

Pathway: Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.One-carbon metabolism; tetrahydrofolate interconversion.

Subunit structure: Homooctamer, including four polyglutamate binding sites. The subunits are arranged as a tetramer of dimers, and form a planar ring-shaped structure

By similarity.

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome › centriole. Golgi apparatus

By similarity. Note: More abundantly located around the mother centriole. Ref.5

Involvement in disease: Glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]: Autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.6

Sequence similarities: In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.In the N-terminal section; belongs to the formiminotransferase family.