Western Blot
(Western Blot analysis using ERK1 antibody1:1000 (1 ug/ml) antibody dilution used in WB of HEK 293 cell lysate; 5 ug (1), 10 ug (2) and 30 ug (3) of cell lysate used. )
Mouse anti-Human, Mouse ERK1 Monoclonal Antibody | anti-ERK1 antibody
ERK1 antibody
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
ERK1 antibody was purified by Protein A affinity chromatography.
Synonyms
ERK1; Monoclonal Antibody; ERK1 antibody; Monoclonal ERK1; Anti-ERK1; ERK 1; ERK-1; P44-ERK1; PRKM3; P44-MAPK; E32; p44; MITOGEN-ACTIVATED PROTEIN KINASE 3; ERK1 Signal Transduction; extracellular signal-regulated kinase 1; anti-ERK1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
E32
Specificity
Human ERK1
Purity/Purification
ERK1 antibody was purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3
Concentration
1 mg/ml (varies by lot)
Sequence Length
422
Applicable Applications for anti-ERK1 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
WB: 1:1000-10,000
Biological Significance
Extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
Cross-Reactivity
Human, mouse
Immunogen
ERK1 antibody was raised in mouse using recombinant full length ERK1 protein as the immunogen.
Preparation and Storage
Store at 4 degree C for several days to weeks. Add 0.1% NaN3 and store at -20 degree C for longer periods. Avoid repeated freeze/thaw cycles.
Western Blot
(Western Blot analysis using ERK1 antibody1:1000 (1 ug/ml) antibody dilution used in WB of HEK 293 cell lysate; 5 ug (1), 10 ug (2) and 30 ug (3) of cell lysate used. )
Western Blot
(Western Blot analysis using ERK1 antibody1:1000 (1 ug/ml) antibody dilution used in WB of HEK 293 cell lysate; 5 ug (1), 10 ug (2) and 30 ug (3) of cell lysate used. )
Related Product Information for anti-ERK1 antibody
Mouse monoclonal ERK1 antibody
Product Categories/Family for anti-ERK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,623 Da
NCBI Official Full Name
Extracellular signal-regulated kinase 1
NCBI Official Symbol
ERK1
NCBI Protein Information
MAP kinase-like orf; aka CEK1; one of two protein kinase genes similar to S. cerevisiae MAP kinases FUS3 (YBL016W) and KSS1 (YGR040W) involved in pheromone signal transduction
UniProt Protein Name
Extracellular signal-regulated kinase 1
UniProt Gene Name
CEK1
UniProt Synonym Gene Names
ERK1; ERK1; MAPK 1
UniProt Entry Name
ERK1_CANAL
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Product Notes
The ERK1 cek1 (Catalog #AAA533158) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ERK1 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). WB: 1:1000-10,000. Researchers should empirically determine the suitability of the ERK1 cek1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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