Flow Cytometry (FC/FACS)
CD155 (PVR) Recombinant Protein | CD155 recombinant protein
CD155 (PVR)-muIg
Gene Names
Pvr; PVS; mE4; HVED; Taa1; CD155; Tage4; necl-5; D7Ertd458e; 3830421F03Rik
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Human CD155-muIg fusion protein was purified from (low FBS containing) tissue culture supernatant of CHO transfectants using Protein A and size exclusion chromatography. Product was 0.2u sterile filtered and vialed under aseptic conditions. Purified (with
Synonyms
CD155 (PVR); CD155 (PVR)-muIg; CD155(PVR)-muIgPurified (with Antibiotics); Human CD155(PVR)-muIg Fusion Protein*; Poliovirus Receptor; CD155 recombinant protein
Purity/Purification
Human CD155-muIg fusion protein was purified from (low FBS containing) tissue culture supernatant of CHO transfectants using Protein A and size exclusion chromatography. Product was 0.2u sterile filtered and vialed under aseptic conditions. Purified (with
Sequence Length
408
Applicable Applications for CD155 recombinant protein
Flow Cytometry (FC/FACS), ELISA (EIA)
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5mg/ml Gentamicin Sulfate (as a preservative).
Performance
Human CD155-muIg is reactive in EIA utilizing GAM capture and detection with anti-CD155 mAb. N-terminal sequencing was as predicted: DVVVQ.
CD155-muIg was tested for FACS binding to 3 day PHA-stimulated human PBMC. Five x 105 cells per tube were washed and princubated 10 minutes with 300ug/ml human Ig (to reduce nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of CD155-muIg at 10 ug/ml. Cells were then washed twice and incubated with 2o detector Goat anti-Mouse/FITC, after which they were washed three times, fixed and analyzed by FACS using a lymphoid gate. Cells stained positive with a mean shift of 0.59 log10 fluorescent units when compared to background.
CD155-muIg was tested for FACS binding to 3 day PHA-stimulated human PBMC. Five x 105 cells per tube were washed and princubated 10 minutes with 300ug/ml human Ig (to reduce nonspecific binding) after which they were incubated 45 minutes on ice with 80 ul of CD155-muIg at 10 ug/ml. Cells were then washed twice and incubated with 2o detector Goat anti-Mouse/FITC, after which they were washed three times, fixed and analyzed by FACS using a lymphoid gate. Cells stained positive with a mean shift of 0.59 log10 fluorescent units when compared to background.
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.
Product should retain activity for at least 6 months after shipping date when stored as recommended.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Related Product Information for CD155 recombinant protein
Human CD155 (Polio Virus Receptor, PVR, Necl-5) is a 70 kd type I Ig superfamily molecule (1).1 It is involved in formation of intracellular junctions between epithelial cells. Its ligands include CD226(DNAM-1), and CD96(TACTILE). CD155 expression by tumor has been shown to be upregulated by Nitric Oxide(2). High CD155 expression has recently been exploited to use engineered poliovirus to treat glioblastoma. (3)
Product Categories/Family for CD155 recombinant protein
References
1) Medelsohn CL, Racaniello VR, et al. (1989) Cell 56(5): 855-65. 2) C Fionda, M Cippitelli, et al. (2015) BMC Cancer 15(1):17 PMID 25609078. 3) Gromeier M, Bigner D, et al. (2014) Neuro-Oncology 16(supp3): iii41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,655 Da
NCBI Official Full Name
cd155
NCBI Official Synonym Full Names
poliovirus receptor
NCBI Official Symbol
Pvr
NCBI Official Synonym Symbols
PVS; mE4; HVED; Taa1; CD155; Tage4; necl-5; D7Ertd458e; 3830421F03Rik
NCBI Protein Information
poliovirus receptor
UniProt Protein Name
Cd155
UniProt Gene Name
Pvr
UniProt Entry Name
Q91WP1_MOUSE
Research Articles on CD155
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Product Notes
The CD155 pvr (Catalog #AAA666919) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD155 (PVR) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the CD155 pvr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD155 (PVR), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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