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Testing Data

B3GNT6 active protein

B3GNT6, soluble fragment, Human Recombinant

Gene Names
B3GNT6; BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
Purity
>95% by SDS-PAGE
Synonyms
B3GNT6; soluble fragment; Human Recombinant; Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1; 3-N acetylglucosaminyltransferase; Core 3 synthase; UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 6; BGnT-6; Beta-1; 3-Gn-T6; B3GNT6 active protein
Ordering
For Research Use Only!
Host
Insect cells
Purity/Purification
>95% by SDS-PAGE
Form/Format
Liquid. Sterile filtered solution in 25mM Tris pH7.5 and 150mM NaCl
Concentration
100ug/ml (varies by lot)
Gene Source
Human
Biological Activity
Specific activity >100 pmol/min/ug
Measured by transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to 4-Nitrophenyl-N-acetylgalactosamine, as measured with a phosphatase-coupled assay.
Preparation and Storage
Store at -20 degree C
Centrifuge the vial prior to opening.
Shelf life: ~12 months

Testing Data

Testing Data
Related Product Information for B3GNT6 active protein
beta-1,3-N-acetylglucosaminyltransferase 6 (beta3GnT6) transfers N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to N-acetylgalactosamine (GalNAc). beta3GnT6 is also known as the core 3 synthase, because it creates the core 3 oligosaccharide structure in the mucin-type O-glycan, GlcNAcbeta1-3GalNAcalpha1-serine/threonine. Core 3 synthase is a type II transmembrane protein, and is localized to stomach, small intestine and colonic tissues. It has long been known that synthesis of the core 3 structure is down-regulated in colonic cancer cells and it has been shown that the expression of beta3GnT6 is also down-regulated. Colonic cancer cells transfected with beta3GnT6 have reduced metastatic potential compared to non-transfected cells, and when injected into mice show decreased tumor formation. Pancreatic cancer cells transfected with beta3GnT6 show a similar phenotype, which has been attributed to the addition of core3 O-glycans to alpha2beta1 integrin.
Product Categories/Family for B3GNT6 active protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
42,748 Da
NCBI Official Full Name
B3GNT6 protein
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6 (core 3 synthase)
NCBI Official Symbol
B3GNT6
NCBI Official Synonym Symbols
BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
NCBI Protein Information
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6; core 3 synthase; beta-1,3-N-acetylglucosaminyltransferase protein
UniProt Protein Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
UniProt Gene Name
B3GNT6
UniProt Synonym Gene Names
BGnT-6; Beta-1,3-Gn-T6; Beta-1,3-N-acetylglucosaminyltransferase 6; Beta3Gn-T6
UniProt Entry Name
B3GN6_HUMAN

Uniprot Description

B3GNT6: Beta-1,3-N-acetylglucosaminyltransferase that synthesizes the core 3 structure of the O-glycan, an important precursor in the biosynthesis of mucin-type glycoproteins. Plays an important role in the synthesis of mucin-type O-glycans in digestive organs. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity; galactosyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; glycoprotein biosynthetic process; O-glycan processing; O-glycan processing, core 3; post-translational protein modification

Research Articles on B3GNT6

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Product Notes

The B3GNT6 b3gnt6 (Catalog #AAA849848) is an Active Protein produced from Insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "B3GNT6, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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