Rabbit anti-Human ALKBH8 Polyclonal Antibody | anti-ALKBH8 antibody

ALKBH8, CT (Alkylated DNA Repair Protein alkB Homolog 8, Probable alpha-ketoglutarate-dependent Dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA Methyltransferase ABH8, ABH8) (PE)

Cat. Num. AAA6462511

• Gene Names: ALKBH8; ABH8; TRM9; TRMT9; TRMT9A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ALKBH8; Polyclonal Antibody; CT (Alkylated DNA Repair Protein alkB Homolog 8; Probable alpha-ketoglutarate-dependent Dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA Methyltransferase ABH8; ABH8) (PE); anti-ALKBH8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ALKBH8.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ALKBH8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ALKBH8 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of ALKBH8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). ALKBH8 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ALKBH8 antibody

Antibodies; DNA Replication; Repair

References

1. Shimada, K., et.al., Cancer Res. 69(7), 3157-3164 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 195927056
• NCBI GeneID: 91801
• NCBI Accession #: NP_620130
• NCBI GenBank Nucleotide #: NM_138775
• UniProt Accession #: Q96BT7
• Molecular Weight: 26kDa
• NCBI Official Full Name: alkylated DNA repair protein alkB homolog 8 isoform 2
• NCBI Official Synonym Full Names: alkB homolog 8, tRNA methyltransferase
• NCBI Official Symbol: ALKBH8
• NCBI Official Synonym Symbols: ABH8; TRM9; TRMT9; TRMT9A
• NCBI Protein Information: alkylated DNA repair protein alkB homolog 8
• UniProt Protein Name: Alkylated DNA repair protein alkB homolog 8
• Protein Family: Alkylated DNA repair protein
• UniProt Gene Name: ALKBH8
• UniProt Synonym Gene Names: ABH8
• UniProt Entry Name: ALKB8_HUMAN

Uniprot Description

ALKBH8: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. Belongs to the alkB family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.229; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; tRNA (uracil) methyltransferase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA binding

Biological Process: tRNA wobble uridine modification; response to DNA damage stimulus; tRNA methylation