Rabbit FACL4/ACSL4 Polyclonal Antibody | anti-ACSL4 antibody

Anti-FACL4/ACSL4 Antibody

Cat. Num. AAA1753107

• Gene Names: ACSL4; ACS4; FACL4; LACS4; MRX63; MRX68
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Immunogen affinity purified.
• Synonyms: FACL4/ACSL4; Polyclonal Antibody; Anti-FACL4/ACSL4 Antibody; Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4; LACS 4; ACSL4; ACS4; FACL4; LACS4; anti-ACSL4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 670

• Applicable Applications for anti-ACSL4 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 0.1-0.25ug/ml (Human, Mouse, Rat); IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
• Immunogen: A synthetic peptide corresponding to a sequence of human FACL4/ACSL4 (EPWTPETGLVTDAFKLKRKELR).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Immunofluorescence (IF)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

Related Product Information for anti-ACSL4 antibody

Description: Rabbit IgG polyclonal antibody for FACL4/ACSL4 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human; Mouse; Rat.

Background: Long-chain-fatty-acid—CoA ligase 4 is an enzyme that in humans is encoded by the ACSL4 gene. It is mapped to Xq23. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

References

1. Piccini M, Vitelli F, Bruttini M, Pober BR, Jonsson JJ, Villanova M, Zollo M, Borsani G, Ballabio A, Renieri A (Apr 1998). "FACL4, a new gene encoding long-chain acyl-CoA synthetase 4, is deleted in a family with Alport syndrome, elliptocytosis, and mental retardation". Genomics. 47 (3): 350-8.
2. Verot L, Alloisio N, Morle L, Bozon M, Touraine R, Plauchu H, Edery P (Sep 2003). "Localization of a non-syndromic X-linked mental retardation gene (MRX80) to Xq22-q24". Am J Med Genet A. 122A (1): 37-41.
3. Zong NC, Li H, Li H, Lam MP, Jimenez RC, Kim CS, Deng N, Kim AK, Choi JH, Zelaya I, Liem D, Meyer D, Odeberg J, Fang C, Lu HJ, Xu T, Weiss J, Duan H, Uhlen M, Yates JR, Apweiler R, Ge J, Hermjakob H, Ping P (Oct 2013). "Integration of cardiac proteome biology and medicine by a specialized knowledgebase". Circulation Research. 113 (9): 1043-53.

NCBI and Uniprot Product Information

• NCBI GI #: 4758332
• NCBI GeneID: 2182
• NCBI Accession #: NP_004449.1
• NCBI GenBank Nucleotide #: NP_004449.1
• UniProt Accession #: O60488
• NCBI Official Full Name: long-chain-fatty-acid--CoA ligase 4 isoform 1
• NCBI Official Synonym Full Names: acyl-CoA synthetase long chain family member 4
• NCBI Official Symbol: ACSL4
• NCBI Official Synonym Symbols: ACS4; FACL4; LACS4; MRX63; MRX68
• NCBI Protein Information: long-chain-fatty-acid--CoA ligase 4
• UniProt Protein Name: Long-chain-fatty-acid--CoA ligase 4
• UniProt Gene Name: ACSL4
• UniProt Synonym Gene Names: ACS4; FACL4; LACS4; LACS 4
• UniProt Entry Name: ACSL4_HUMAN

NCBI Description

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

ACSL4: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. Defects in ACSL4 are the cause of mental retardation X- linked type 63 (MRX63). Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non- syndromic mental retardation patients do not manifest other clinical signs. Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR). A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.2.1.3; Membrane protein, integral; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: Xq22.3-q23

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; endoplasmic reticulum membrane; cell soma; membrane; cytoplasm; integral to membrane; lipid particle

Molecular Function: arachidonate-CoA ligase activity; very-long-chain-fatty-acid-CoA ligase activity; ATP binding; long-chain-fatty-acid-CoA ligase activity

Biological Process: embryonic process involved in female pregnancy; lipid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; lipid metabolic process; long-chain fatty acid metabolic process; negative regulation of prostaglandin secretion; positive regulation of cell growth; response to nutrient; fatty acid transport

Disease: Mental Retardation, X-linked 63