Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hepg2, hela and 293T cell, human fetal kidney tissue Amount of lysate: 40ug per lane Primary antibody: 1/225 dilution Secondary antibody dilution: 1/8000 Exposure time: 15 seconds)
Rabbit anti-Human ACSL4 Polyclonal Antibody | anti-ACSL4 antibody
ACSL4 Antibody
Immunohistochemistry: 1:25-1:100
Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hepg2, hela and 293T cell, human fetal kidney tissue Amount of lysate: 40ug per lane Primary antibody: 1/225 dilution Secondary antibody dilution: 1/8000 Exposure time: 15 seconds)
Testing Data
(Gel: 8%SDS-PAGE Lysates (from left to right): Hepg2, hela and 293T cell, human fetal kidney tissue Amount of lysate: 40ug per lane Primary antibody: 1/225 dilution Secondary antibody dilution: 1/8000 Exposure time: 15 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/25.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/25.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
ACSL4: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. Defects in ACSL4 are the cause of mental retardation X- linked type 63 (MRX63). Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non- syndromic mental retardation patients do not manifest other clinical signs. Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR). A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Ligase; Lipid Metabolism - fatty acid; EC 6.2.1.3
Chromosomal Location of Human Ortholog: Xq22.3-q23
Cellular Component: peroxisomal membrane; mitochondrial outer membrane; endoplasmic reticulum membrane; cell soma; membrane; cytoplasm; integral to membrane; lipid particle
Molecular Function: arachidonate-CoA ligase activity; very-long-chain-fatty-acid-CoA ligase activity; ATP binding; long-chain-fatty-acid-CoA ligase activity
Biological Process: lipid biosynthetic process; embryonic process involved in female pregnancy; triacylglycerol biosynthetic process; cellular lipid metabolic process; long-chain fatty acid metabolic process; lipid metabolic process; negative regulation of prostaglandin secretion; positive regulation of cell growth; response to nutrient; fatty acid transport
Disease: Mental Retardation, X-linked 63
Research Articles on ACSL4
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Product Notes
The ACSL4 acsl4 (Catalog #AAA9409862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACSL4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ACSL4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:500-1:2000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the ACSL4 acsl4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACSL4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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