Rabbit anti-Human ACSL4 Polyclonal Antibody | anti-ACSL4 antibody

ACSL4, NT (ACSL4, ACS4, FACL4, LACS4, Long-chain-fatty-acid-CoA ligase 4, Long-chain acyl-CoA synthetase 4) (APC)

Cat. Num. AAA6271124

• Gene Names: ACSL4; ACS4; FACL4; LACS4; MRX63; MRX68
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ACSL4; Polyclonal Antibody; NT (ACSL4; ACS4; FACL4; LACS4; Long-chain-fatty-acid-CoA ligase 4; Long-chain acyl-CoA synthetase 4) (APC); Long-chain-fatty-acid--CoA ligase 4; Long-chain acyl-CoA synthetase 4; anti-ACSL4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 711

• Applicable Applications for anti-ACSL4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ACSL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human ACSL4.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ACSL4 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ACSL4 antibody detected the ACSL4 protein (arrow).)

Related Product Information for anti-ACSL4 antibody

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants.

Product Categories/Family for anti-ACSL4 antibody

Antibodies; Enzymes; Synthase

References

Bosker, F.J., et al. Mol. Psychiatry (2010). In press: Zhang, Y., et al. Hum. Mol. Genet. 18(20):3894-3905(2009). Zeman, M., et al. Tohoku J. Exp. Med. 217(4):287-293(2009). An, C., et al. Neurosci. Lett. 441(2):197-200(2008). Hu, C., et al. Cancer Biol. Ther. 7(1):131-134(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 12669909
• NCBI GeneID: 2182
• NCBI Accession #: NP_075266.1; NP_004449.1
• NCBI GenBank Nucleotide #: NP_075266.1
• UniProt Accession #: O60488
• NCBI Official Full Name: long-chain-fatty-acid--CoA ligase 4 isoform 2
• NCBI Official Synonym Full Names: acyl-CoA synthetase long chain family member 4
• NCBI Official Symbol: ACSL4
• NCBI Official Synonym Symbols: ACS4; FACL4; LACS4; MRX63; MRX68
• NCBI Protein Information: long-chain-fatty-acid--CoA ligase 4
• UniProt Protein Name: Long-chain-fatty-acid--CoA ligase 4
• UniProt Gene Name: ACSL4
• UniProt Synonym Gene Names: ACS4; FACL4; LACS4; LACS 4
• UniProt Entry Name: ACSL4_HUMAN

NCBI Description

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

ACSL4: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. Defects in ACSL4 are the cause of mental retardation X- linked type 63 (MRX63). Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non- syndromic mental retardation patients do not manifest other clinical signs. Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR). A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.2.1.3; Membrane protein, integral; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: Xq22.3-q23

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; endoplasmic reticulum membrane; cell soma; membrane; cytoplasm; integral to membrane; lipid particle

Molecular Function: arachidonate-CoA ligase activity; very-long-chain-fatty-acid-CoA ligase activity; ATP binding; long-chain-fatty-acid-CoA ligase activity

Biological Process: embryonic process involved in female pregnancy; lipid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; lipid metabolic process; long-chain fatty acid metabolic process; negative regulation of prostaglandin secretion; positive regulation of cell growth; response to nutrient; fatty acid transport

Disease: Mental Retardation, X-linked 63