Rabbit ETAR Polyclonal Antibody | anti-ETAR antibody

ETAR (N427) Polyclonal Antibody

Cat. Num. AAA3002827

• Gene Names: EDNRA; ETA; ET-A; ETAR; ETRA; MFDA; ETA-R; hET-AR
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ETAR; Polyclonal Antibody; ETAR (N427) Polyclonal Antibody; Endothelin-1 receptor; Endothelin A receptor; ET-A; ETA-R; hET-AR; EDNRA; ETA; ETRA; anti-ETAR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ETAR (N427) polyclonal antibody detects endogenous levels of ETAR protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 318

• Applicable Applications for anti-ETAR antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 402-452 of Human ETAR.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ETAR (N427) pAb at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug))

Related Product Information for anti-ETAR antibody

Endothelin receptor A (ETAR), also known as EDNRA, ET1 receptor, ETA, EDN1 and ET-AR, is a member of the guanine-binding regulatory protein-coupled receptor family. ETAR binds endothelins and has the highest affinity for its ligand, ET1, as compared to the ETBR receptor. Both ET receptors, ETAR and ETBR, are activated by ET1, which results in inhibition of active lens sodiumpotassium transport. Activation of the ET receptors also causes an increase in cytoplasmic calcium concentration in cultured lens epithelial cells. In addition, ETAR induces arachidonic acid accumulation. ETAR has seven hydrophobic transmembrane domains and is expressed in aorta, lung, atrium, kidney, placenta and prostate. Specifically, placental vascular smooth muscle cells (PVSMCs) exclusively express ETAR.

NCBI and Uniprot Product Information

• NCBI GI #: 260593650
• NCBI GeneID: 1909
• NCBI Accession #: NP_001159527.1
• NCBI GenBank Nucleotide #: NP_001159527.1
• UniProt Accession #: P25101
• Molecular Weight: ~ 50kDa
• NCBI Official Full Name: endothelin-1 receptor isoform b
• NCBI Official Synonym Full Names: endothelin receptor type A
• NCBI Official Symbol: EDNRA
• NCBI Official Synonym Symbols: ETA; ET-A; ETAR; ETRA; MFDA; ETA-R; hET-AR
• NCBI Protein Information: endothelin-1 receptor
• UniProt Protein Name: Endothelin-1 receptor
• UniProt Gene Name: EDNRA
• UniProt Synonym Gene Names: ETA; ETRA; ET-A; ETA-R; hET-AR
• UniProt Entry Name: EDNRA_HUMAN

NCBI Description

This gene encodes the receptor for endothelin-1, a peptide that plays a role in potent and long-lasting vasoconstriction. This receptor associates with guanine-nucleotide-binding (G) proteins, and this coupling activates a phosphatidylinositol-calcium second messenger system. Polymorphisms in this gene have been linked to migraine headache resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

EDNRA: Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1

Chromosomal Location of Human Ortholog: 4q31.22

Cellular Component: nuclear membrane; integral to plasma membrane; T-tubule; plasma membrane; lipid raft

Molecular Function: endothelin receptor activity; protein binding; phosphoinositide phospholipase C activity

Biological Process: positive regulation of odontogenesis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); neural crest cell development; metabolic process; heart development; response to morphine; smooth muscle cell proliferation; response to lipopolysaccharide; glucose transport; signal transduction; sensory perception of pain; enteric nervous system development; regulation of epithelial cell proliferation; Rho protein signal transduction; histamine secretion; elevation of cytosolic calcium ion concentration; negative regulation of cAMP biosynthetic process; smooth muscle contraction; fibroblast proliferation; regulation of blood pressure; protein kinase C activation; positive regulation of cell proliferation; artery smooth muscle contraction; penile erection; aging; vasoconstriction; in utero embryonic development; glomerular filtration; adenylate cyclase activation; respiratory gaseous exchange; patterning of blood vessels; cell proliferation; G-protein coupled receptor protein signaling pathway; phospholipase C activation; maternal process involved in parturition; response to hypoxia; positive regulation of protein amino acid phosphorylation; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of inflammatory response; negative regulation of apoptosis

Disease: Mandibulofacial Dysostosis With Alopecia; Migraine With Or Without Aura, Susceptibility To, 1