Rabbit EPAC1 Polyclonal Antibody | anti-EPAC1 antibody

EPAC1 Antibody

Cat. Num. AAA9410538

• Gene Names: Rapgef3; Epac; Epac1; 2310016P22Rik; 9330170P05Rik
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity chromatography purified via peptide column
• Synonyms: EPAC1; Polyclonal Antibody; EPAC1 Antibody; Rap guanine nuclear exchange factor 3; RAPGEF3; cAMPGEF-I; bcm910; anti-EPAC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 918

• Applicable Applications for anti-EPAC1 antibody: ELISA (EIA), Western Blot (WB)
• Immunogen Type: Peptide
• Immunogen Description: Raised against an 18 amino acid peptide near the amino terminus of human EPAC1.
• Target Name: EPAC1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of EPAC1 in rat skeletal muscle tissue lysate with EPAC1 antibody at 1 ug/mL.)

Related Product Information for anti-EPAC1 antibody

EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.

Product Categories/Family for anti-EPAC1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 295317403
• NCBI GeneID: 223864
• NCBI Accession #: NP_659099
• NCBI GenBank Nucleotide #: NM_144850.2
• Molecular Weight: 104,426 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 3 isoform 2
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 3
• NCBI Official Symbol: Rapgef3
• NCBI Official Synonym Symbols: Epac; Epac1; 2310016P22Rik; 9330170P05Rik
• NCBI Protein Information: rap guanine nucleotide exchange factor 3
• UniProt Protein Name: Rap guanine nucleotide exchange factor 3
• UniProt Gene Name: Rapgef3
• UniProt Synonym Gene Names: Epac; Epac1; EPAC 1; cAMP-GEFI
• UniProt Entry Name: RPGF3_MOUSE

Uniprot Description

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Cellular Component: membrane; cytoplasm; intracellular; cAMP-dependent protein kinase complex

Molecular Function: Rap guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; nucleotide binding; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; positive regulation of protein export from nucleus; positive regulation of angiogenesis; regulation of protein amino acid phosphorylation; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of stress fiber formation; angiogenesis; positive regulation of peptidyl-serine phosphorylation; Rap protein signal transduction; positive regulation of GTPase activity