Rabbit NEP / DDR1 Polyclonal Antibody | anti-NEP antibody

Rabbit Polyclonal to Human NEP / DDR1

Cat. Num. AAA247303

• Gene Names: DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
• Reactivity: Gibbon, Chimpanzee, Gorilla, Horse, Human, Monkey; Predicted Reactivity: Bovine, Pig, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: NEP / DDR1; Polyclonal Antibody; Rabbit Polyclonal to Human NEP / DDR1; Anti-NEP / DDR1 Antibody (Internal) IHC-plus; DDR1; Cell adhesion kinase; CD167; CD167a antigen; EDDR1; ENTRK4; Mammary carcinoma kinase 10; MCK10; NEP; PTK3; PTK3A; RTK6; Tyrosine-protein kinase CAK; CAK; DDR; Discoidin domain receptor 1; HGK2; MCK-10; NTRK4; Protein-tyrosine kinase 3A; Human NEP; anti-NEP antibody

• Host: Rabbit
• Reactivity: Gibbon, Chimpanzee, Gorilla, Horse, Human, Monkey; Predicted Reactivity: Bovine, Pig, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human DDR1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 919

• Applicable Applications for anti-NEP antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (2 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 16 amino acid peptide from internal region of human DDR1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Elephant, Horse (100%); Bovine, Rabbit, Pig (94%); Monkey, Panda (88%).
• Immunogen Type: Synthetic peptide
• Immunogen: NEP / DDR1 antibody was raised against synthetic 16 amino acid peptide from internal region of human DDR1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Elephant, Horse (100%); Bovine, Rabbit, Pig (94%); Monkey, Panda (88%); Dog, Bat, Opossum, Platypus (81%).
• Antigen Modification: Internal
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-DDR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-NEP antibody

DDR1 is a DDR/TKT type protein kinase. DDR1 is activated by various types of collagen, including types I through IV. Binding of collagen to DDR1 protein results in autophosphorylation and a delayed but sustained tyrosine kinase activation. DDR1 may function in cell-to-cell interaction or recognition. At least three mRNA variants, resulting in different protein isoforms of 875, 913 and 919 amino acids, have been reported.

Product Categories/Family for anti-NEP antibody

Family: Protein Kinase; Subfamily: DDR/TKT

NCBI and Uniprot Product Information

• NCBI GI #: 83977452
• NCBI GeneID: 780
• NCBI Accession #: NP_054700.2
• NCBI GenBank Nucleotide #: NM_013994.2
• UniProt Accession #: Q08345; Q14196; Q16562; Q2L6H3; Q4LE50; Q5ST11; Q5ST12; Q6NSK4; Q9UD35; B5A975; B5A976; B7Z2K0
• Molecular Weight: 99,064 Da
• NCBI Official Full Name: epithelial discoidin domain-containing receptor 1 isoform 3
• NCBI Official Synonym Full Names: discoidin domain receptor tyrosine kinase 1
• NCBI Official Symbol: DDR1
• NCBI Official Synonym Symbols: CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A
• NCBI Protein Information: epithelial discoidin domain-containing receptor 1; tyrosine kinase DDR; cell adhesion kinase; mammary carcinoma kinase 10; tyrosine-protein kinase CAK; protein-tyrosine kinase RTK-6; neuroepithelial tyrosine kinase; PTK3A protein tyrosine kinase 3A; CD167 antigen-like family member A; neurotrophic tyrosine kinase, receptor, type 4
• UniProt Protein Name: Epithelial discoidin domain-containing receptor 1
• Protein Family: Halolysin-like extracellular serine protease
• UniProt Gene Name: DDR1
• UniProt Synonym Gene Names: CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; Epithelial discoidin domain receptor 1; MCK-10
• UniProt Entry Name: DDR1_HUMAN

NCBI Description

Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

DDR1: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2. Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells. Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Protein kinase, TK; Kinase, protein; TK group; DDR family

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: collagen binding; protein binding; metal ion binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: lactation; negative regulation of cell proliferation; extracellular matrix organization and biogenesis; regulation of cell-matrix adhesion; protein amino acid autophosphorylation; smooth muscle cell migration; ear development; regulation of cell growth; cell adhesion; embryo implantation