Rabbit EPAC1 Polyclonal Antibody | anti-RAPGEF3 antibody

EPAC1 Antibody

Cat. Num. AAA150164

• Gene Names: RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: EPAC1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: EPAC1; Polyclonal Antibody; EPAC1 Antibody; EPAC; bcm910; HSU79275; CAMP-GEFI; CGEF1; Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; EPAC 1; Rap guanine nucleotide exchange factor (GEF) 3; anti-RAPGEF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.
• Purity/Purification: EPAC1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 923

• Applicable Applications for anti-RAPGEF3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: EPAC1 antibody can be used for detection of EPAC1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: EPAC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EPAC1.
• Buffer: EPAC1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: EPAC1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of EPAC1 in rat skeletal muscle tissue lysate with EPAC1 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of EPAC1 in rat brain tissue with EPAC1 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of EPAC1 in rat brain tissue with EPAC1 antibody at 20 μg/mL.)

Related Product Information for anti-RAPGEF3 antibody

EPAC1 Antibody: EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.

NCBI and Uniprot Product Information

• NCBI GI #: 148747859
• NCBI GeneID: 10411
• NCBI Accession #: NP_659099
• NCBI GenBank Nucleotide #: NM_001098531.2
• UniProt Accession #: O95398; O95634; Q8WVN0; A8K2G5; E7EQC8
• Molecular Weight: 99,452 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 3 isoform a
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 3
• NCBI Official Symbol: RAPGEF3
• NCBI Official Synonym Symbols: EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI
• NCBI Protein Information: rap guanine nucleotide exchange factor 3; EPAC 1; 9330170P05Rik; exchange factor directly activated by cAMP 1; exchange protein directly activated by cAMP 1; cAMP-regulated guanine nucleotide exchange factor I; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP
• UniProt Protein Name: Rap guanine nucleotide exchange factor 3
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF3
• UniProt Synonym Gene Names: CGEF1; EPAC; EPAC1; EPAC 1; cAMP-GEFI
• UniProt Entry Name: RPGF3_HUMAN

Uniprot Description

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: membrane; plasma membrane; endomembrane system; cAMP-dependent protein kinase complex

Molecular Function: Rap guanyl-nucleotide exchange factor activity; protein binding; guanyl-nucleotide exchange factor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; platelet activation; signal transduction; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein export from nucleus; cell proliferation; cAMP-mediated signaling; positive regulation of angiogenesis; positive regulation of stress fiber formation; energy reserve metabolic process; positive regulation of cAMP catabolic process; angiogenesis; blood coagulation; regulation of protein kinase activity; regulation of insulin secretion; Rap protein signal transduction