Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protoporphyrinogen oxidase (PPOX) Standard Curve.)
Mouse Protoporphyrinogen Oxidase (PPOX) ELISA Kit | PPOX elisa kit
Mouse Protoporphyrinogen Oxidase (PPOX) ELISA Kit
Gene Names
Ppox; Ppo
Reactivity
Mouse
Synonyms
Protoporphyrinogen Oxidase (PPOX); Mouse Protoporphyrinogen Oxidase (PPOX) ELISA Kit; PPOX; MGC8485; PPO; V290M; VP; PPOX elisa kit
Reactivity
Mouse
Specificity
Mouse Protoporphyrinogen oxidase (PPOX) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPOX. No significant cross-reactivity or interference between Mouse PPOX and analogues was observed.
Sequence Length
477
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protoporphyrinogen oxidase (PPOX) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protoporphyrinogen oxidase (PPOX) Standard Curve.)
Related Product Information for PPOX elisa kit
Description: This Mouse Protoporphyrinogen oxidase (PPOX) ELISA Kit employs a two-site sandwich ELISA to quantitate PPOX in samples. An antibody specific for PPOX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPOX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPOX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPOX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PPOX is a human gene that produces an enzyme called protoporphyrinogen oxidase. This enzyme is responsible for the seventh step in heme production. Heme is the portion of hemoglobin that carries oxygen in the blood from the lungs to the rest of the body. Each of the steps in heme production is controlled by a separate gene. Protoporphyrinogen oxidase removes hydrogen atoms from protoporphyrinogen IX (the product of the sixth step in the production of heme) to form protoporphyrin IX. One additional enzyme must modify protoporphyrin IX before it becomes heme.This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. This protein is a flavoprotein associated with the outer surface of the inner mitochondrial membrane.
Background: PPOX is a human gene that produces an enzyme called protoporphyrinogen oxidase. This enzyme is responsible for the seventh step in heme production. Heme is the portion of hemoglobin that carries oxygen in the blood from the lungs to the rest of the body. Each of the steps in heme production is controlled by a separate gene. Protoporphyrinogen oxidase removes hydrogen atoms from protoporphyrinogen IX (the product of the sixth step in the production of heme) to form protoporphyrin IX. One additional enzyme must modify protoporphyrin IX before it becomes heme.This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. This protein is a flavoprotein associated with the outer surface of the inner mitochondrial membrane.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protoporphyrinogen oxidase
NCBI Official Synonym Full Names
protoporphyrinogen oxidase
NCBI Official Symbol
Ppox
NCBI Official Synonym Symbols
Ppo
NCBI Protein Information
protoporphyrinogen oxidase
UniProt Protein Name
Protoporphyrinogen oxidase
UniProt Gene Name
Ppox
UniProt Synonym Gene Names
PPO
UniProt Entry Name
PPOX_MOUSE
Research Articles on PPOX
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Product Notes
The Mouse PPOX ppox (Catalog #AAA9717312) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717312 ELISA Kit recognizes Mouse PPOX. It is sometimes possible for the material contained within the vial of "Protoporphyrinogen Oxidase (PPOX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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