Rabbit EGF Polyclonal Antibody | anti-EGF antibody

RABBIT ANTI HUMAN EGF

Cat. Num. AAA221706

• Gene Names: EGF; URG; HOMG4
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: EGF; Polyclonal Antibody; RABBIT ANTI HUMAN EGF; anti-EGF antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 1166

• Applicable Applications for anti-EGF antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: This purified human EGF antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human EGF antibody as the detection reagent and recombinant human EGF as the standard.; Western Blot: Rabbit anti human EGF may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human EGF as the positive control.; ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml; Functional Assays: Maximum Dilution: <0.1ug/ml; Immunohistology - Paraffin: Maximum Dilution: 5.0ug/ml; Application Note: This product requires heat-mediated pre-treatment of paraffin sections prior to staining. Citrate buffer pH 6.0 is recommended for this purpose; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add azide.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
• Immunogen: Recombinant human EGF.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (MBS221706) following emzyme mediated antigen retrieval using proteinase K)

Testing Data #2

(Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human EGF (MBS221706) following emzyme mediated antigen retrieval using proteinase K)

Related Product Information for anti-EGF antibody

Rabbit anti human EGF recognizes human Epidermal Growth Factor, a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, which is expressed by a variety of cells.. The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the 170kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-a, VGF (vaccinia virus growth factor) and Betacellulin, produced by certain tumour cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 296011013
• NCBI GeneID: 1950
• NCBI Accession #: NP_001171601.1
• NCBI GenBank Nucleotide #: NM_001178130.1
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: 129,746 Da
• NCBI Official Full Name: pro-epidermal growth factor isoform 2 preproprotein
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor; beta-urogastrone
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN

NCBI Description

This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]

Uniprot Description

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral; Hormone

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; lysosomal membrane; extracellular region; plasma membrane; integral to membrane

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase activation (dimerization); nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; angiogenesis; DNA replication; positive regulation of DNA binding; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of mitosis; branching morphogenesis of a tube; innate immune response; negative regulation of secretion; blood coagulation; positive regulation of phosphorylation

Disease: Hypomagnesemia 4, Renal