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Testing Data #1
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Goat DEFENSIN BETA 2 Polyclonal Antibody | anti-DEFB4B antibody
GOAT ANTI HUMAN DEFENSIN BETA 2
Gene Names
DEFB4B; DEFB4P
Applications
ELISA, Functional Assay, Immunohistochemistry, Western Blot
Synonyms
DEFENSIN BETA 2; Polyclonal Antibody; GOAT ANTI HUMAN DEFENSIN BETA 2; anti-DEFB4B antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Form/Format
Purified
Purified IgG - lyophilised
Purified IgG - lyophilised
Concentration
IgG concentration 0.1 mg/ml after reconstitution (varies by lot)
Sequence Length
64
Applicable Applications for anti-DEFB4B antibody
ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin, Western Blot (WB)
Application Notes
ELISA: This purified human BD-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human BD-2 antibody as the detection reagent and recombinant human BD-2 as the standard
Western Blot: This purified human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 as the positive control.
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml
Functional Assays: Minimum Dilution: 5.0; Maximum Dilution: 8.0ug/ml
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
Western Blot: This purified human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 as the positive control.
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml
Functional Assays: Minimum Dilution: 5.0; Maximum Dilution: 8.0ug/ml
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
Reconstitution
Reconstitute with 1.0ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Preparation
Preparation
Immunogen
Recombinant human Defensin beta-2.
Buffer Solution
Buffer Solution
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Testing Data #1
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Testing Data #1
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Testing Data #2
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Testing Data #2
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Testing Data #3
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Testing Data #3
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Testing Data #4
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Testing Data #4
(Staining of formalin fixed, paraffin embedded inflamed oral mucosa with Goat anti Human beta defensin-1 (MBS221749))
Related Product Information for anti-DEFB4B antibody
Goat anti Human Defensin beta 2 polyclonal antibody recognizes human Defensin beta-2 (BD-2), a ~4.3kDa cationic antimicrobial peptide, also known as Beta-defensin 4A or Skin-antimicrobial peptide 1 (SAP-1) expressed primarily by epithelial cells of the respiratory tract and skin, and an important component of the innate immune response against microbial infections. Expression of BD-2 is induced during inflammation in response to bacterial products and cytokines, and is initially expressed in a precursor form, which is cleaved to release the C-Terminal active portion of the protein which is secreted by the neutrophil and binds to bacterial membranes causing their disruption.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,038 Da
NCBI Official Full Name
beta-defensin 4B
NCBI Official Synonym Full Names
defensin, beta 4B
NCBI Official Symbol
DEFB4B
NCBI Official Synonym Symbols
DEFB4P
NCBI Protein Information
beta-defensin 4B; beta defensin 2; beta defensin-2; beta-defensin 2; defensin, beta 4, pseudogene
UniProt Protein Name
Beta-defensin 4A
UniProt Gene Name
DEFB4A
UniProt Synonym Gene Names
DEFB102; DEFB2; DEFB4; BD-2; hBD-2; SAP1
UniProt Entry Name
DFB4A_HUMAN
NCBI Description
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Oct 2014]
Uniprot Description
defensin, beta 2: Has antibacterial activity (Potential). Belongs to the beta-defensin family. LAP/TAP subfamily.
Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: Golgi lumen; extracellular region
Biological Process: G-protein coupled receptor protein signaling pathway; defense response to bacterium; innate immune response; immune response; chemotaxis
Research Articles on DEFB4B
Similar Products
Product Notes
The DEFB4B defb4a (Catalog #AAA221749) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DEFENSIN BETA 2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin, Western Blot (WB). ELISA: This purified human BD-2 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human BD-2 antibody as the detection reagent and recombinant human BD-2 as the standard Western Blot: This purified human BD-2 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human BD-2 as the positive control. ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml Functional Assays: Minimum Dilution: 5.0; Maximum Dilution: 8.0ug/ml Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml. Researchers should empirically determine the suitability of the DEFB4B defb4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEFENSIN BETA 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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