Rabbit IGF-II Polyclonal Antibody | anti-IGF2 antibody

RABBIT ANTI HUMAN INSULIN-LIKE GROWTH FACTOR II

Cat. Num. AAA221715

• Gene Names: IGF2; IGF-II; PP9974; C11orf43
• Applications: ELISA, Functional Assay, Western Blot
• Synonyms: IGF-II; Polyclonal Antibody; RABBIT ANTI HUMAN INSULIN-LIKE GROWTH FACTOR II; anti-IGF2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 180

• Applicable Applications for anti-IGF2 antibody: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: This purified human IGF-II antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IGF-II antibody as the detection reagent and recombinant human IGF-II as the standard.; Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IGF-II as the positive control.; ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml; Functional Assays: Minimum Dilution: 3.1; Maximum Dilution: 4.6ug/ml; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human IGF-II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of formalin fixed, paraffin embedded normal human placenta with Rabbit anti Human insulin-like growth factor II (MBS221715))

Testing Data #2

(Staining of formalin fixed, paraffin embedded normal human placenta with Rabbit anti Human insulin-like growth factor II (MBS221715))

Testing Data #3

(Staining of formalin fixed, paraffin embedded normal human placenta with Rabbit anti Human insulin-like growth factor II (MBS221715))

Related Product Information for anti-IGF2 antibody

Rabbit anti Human Insulin-Like Growth Factor II antibody recognizes human IGF-II (Insulin-like Growth Factor II), a 67 amino acid mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen. IGF-II is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulin-like growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR). The availability of IGF-II is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research (Singer et al. 2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4504609
• NCBI GeneID: 3481
• NCBI Accession #: NP_000603.1
• NCBI GenBank Nucleotide #: NM_000612.5
• UniProt Accession #: P01344; P78449; Q14299; Q1WM26; Q9UC68; Q9UC69; B3KX48; B7WP08; C9JAF2; E3UN45
• Molecular Weight: 26,331 Da
• NCBI Official Full Name: insulin-like growth factor II isoform 1 preproprotein
• NCBI Official Synonym Full Names: insulin-like growth factor 2
• NCBI Official Symbol: IGF2
• NCBI Official Synonym Symbols: IGF-II; PP9974; C11orf43
• NCBI Protein Information: insulin-like growth factor II; T3M-11-derived growth factor; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor type 2
• UniProt Protein Name: Insulin-like growth factor II
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF2
• UniProt Synonym Gene Names: IGF-II
• UniProt Entry Name: IGF2_HUMAN

NCBI Description

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: protein serine/threonine kinase activator activity; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; receptor activator activity; insulin receptor binding

Biological Process: positive regulation of catalytic activity; response to nicotine; genetic imprinting; multicellular organismal development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; female pregnancy; positive regulation of activated T cell proliferation; response to estradiol stimulus; response to radiation; platelet degranulation; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; skeletal development; response to nutrient levels; response to drug; platelet activation; positive regulation of mitosis; glucose metabolic process; memory; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; cellular protein metabolic process; response to ethanol; insulin receptor signaling pathway; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of insulin receptor signaling pathway; blood coagulation

Disease: Silver-russell Syndrome; Growth Restriction, Severe, With Distinctive Facies; Beckwith-wiedemann Syndrome; Wilms Tumor 1