Rabbit IL-17 Polyclonal Antibody | anti-IL17A antibody

RABBIT ANTI HUMAN INTERLEUKIN-17

Cat. Num. AAA221587

• Gene Names: IL17A; IL17; CTLA8; IL-17; IL-17A
• Applications: ELISA, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: IL-17; Polyclonal Antibody; RABBIT ANTI HUMAN INTERLEUKIN-17; anti-IL17A antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0 mg/ml after reconstitution (varies by lot)
• Sequence Length: 155

• Applicable Applications for anti-IL17A antibody: ELISA (EIA), Functional Assays (FN)*, Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: This purified human IL-17 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our biotinylated human IL-17 antibody as the detection reagent and recombinant human IL-17 as the standard.; Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human IL-17 as the positive control.; ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml; Functional Assays: Minimum Dilution: 0.93; Maximum Dilution: 1.3ug/ml; Application Note: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-17A (50.0 ng/ml).; Immunohistology - Paraffin: Maximum Dilution: 0.25ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human IL-17 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of formalin fixed,paraffin embedded human breast invasive ductal carcinoma with Rabbit anti Human interleukin-17 (MBS221587))

Testing Data #2

(Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Goat anti Human IL-17A (MBS221587) following heat mediated antigen retrieval with sodium citrate buffer (pH6.0))

Related Product Information for anti-IL17A antibody

Rabbit anti Human Interleukin-17 antibody recognizes human IL-17 (Interleukin-17/17A), a 32kDa disulfide-linked homodimer consisting of two 136 amino acid chains, which belongs to the IL-17 family (classified as IL-17A to IL-17F), and signals through the IL-17 receptor (IL-17R/CDw217). IL-17 is produced primarily by activated CD4+ T cells and induces a variety of target cells to secrete inflammatory cytokines, including IL-1beta, IL-6, TNFalpha, IFNgamma and granulocyte colony-stimulating factor, as well as synergizing with other inflammatory cytokines and agonists. The role of IL-17 in bone metabolism has become a focus area for studies into Rheumatoid Arthritis, in which levels of IL-17 are significantly increased and may contribute to bone destruction.

NCBI and Uniprot Product Information

• NCBI GI #: 4504651
• NCBI GeneID: 3605
• NCBI Accession #: NP_002181.1
• NCBI GenBank Nucleotide #: NM_002190.2
• UniProt Accession #: Q16552; Q5T2P0
• Molecular Weight: 17,504 Da
• NCBI Official Full Name: interleukin-17A
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: IL17A
• NCBI Official Synonym Symbols: IL17; CTLA8; IL-17; IL-17A
• NCBI Protein Information: interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• UniProt Protein Name: Interleukin-17A
• Protein Family: Interleukin
• UniProt Gene Name: IL17A
• UniProt Synonym Gene Names: CTLA8; IL17; IL-17; IL-17A; CTLA-8
• UniProt Entry Name: IL17_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: cytokine activity

Biological Process: cell death; cell-cell signaling; apoptosis; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; protein amino acid glycosylation; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response