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Western Blot (WB) (Western Blot (WB) analysis of EEF1D polyclonal antibody)

Rabbit EEF1D Polyclonal Antibody | anti-EEF1D antibody

EEF1D Polyclonal Antibody

Gene Names
EEF1D; EF1D; EF-1D; FP1047
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
EEF1D; Polyclonal Antibody; EEF1D Polyclonal Antibody; Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; Eukaryotic translation elongation factor 1 delta; Guanine nucleotide exchange protein; anti-EEF1D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
EEF1D polyclonal antibody detects endogenous levels of EEF1D protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
281
Applicable Applications for anti-EEF1D antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human EEF1D.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of EEF1D polyclonal antibody)

Western Blot (WB) (Western Blot (WB) analysis of EEF1D polyclonal antibody)
Related Product Information for anti-EEF1D antibody
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~38kDa
NCBI Official Full Name
Elongation factor 1-delta
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 delta
NCBI Official Symbol
EEF1D
NCBI Official Synonym Symbols
EF1D; EF-1D; FP1047
NCBI Protein Information
elongation factor 1-delta
UniProt Protein Name
Elongation factor 1-delta
Protein Family
UniProt Gene Name
EEF1D
UniProt Synonym Gene Names
EF1D; EF-1-delta
UniProt Entry Name
EF1D_HUMAN

NCBI Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]

Uniprot Description

EEF1D: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: eukaryotic translation elongation factor 1 complex; endoplasmic reticulum; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: signal transducer activity; protein binding; transcription activator binding; DNA binding; translation factor activity, nucleic acid binding; heat shock protein binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; translation; transcription, DNA-dependent; mRNA transcription; gene expression; signal transduction

Research Articles on EEF1D

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Product Notes

The EEF1D eef1d (Catalog #AAA3011015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EEF1D Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the EEF1D eef1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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