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Western Blot (WB) (Western Blot analysis of EEF1D in A549, A172 and HepG2 cell lysates in order using EEF1D Rabbit pAb at dilution 1/950)

Rabbit EEF1D Polyclonal Antibody | anti-EEF1D antibody

EEF1D Rabbit pAb

Gene Names
EEF1D; EF1D; EF-1D; FP1047
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
EEF1D; Polyclonal Antibody; EEF1D Rabbit pAb; eukaryotic translation elongation factor 1 delta; EF1D; EF-1D; FP1047; anti-EEF1D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
1.14mg/ml (varies by lot)
Applicable Applications for anti-EEF1D antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:1000-1:5000
IHC-P: 1:50-1:300
Immunogen
Fusion protein of human EEF1D
Conjugation
Unconjugated
Modification
Unmodified
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of EEF1D in A549, A172 and HepG2 cell lysates in order using EEF1D Rabbit pAb at dilution 1/950)

Western Blot (WB) (Western Blot analysis of EEF1D in A549, A172 and HepG2 cell lysates in order using EEF1D Rabbit pAb at dilution 1/950)

Immunohistochemistry (IHC)

(Immunohistochemistry of EEF1D in paraffin-embedded Human thyroid cancer tissue using EEF1D Rabbit pAb at dilution 1/65. Predicted cell location: Cytoplasm)

Immunohistochemistry (IHC) (Immunohistochemistry of EEF1D in paraffin-embedded Human thyroid cancer tissue using EEF1D Rabbit pAb at dilution 1/65. Predicted cell location: Cytoplasm)
Related Product Information for anti-EEF1D antibody
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
281
NCBI Official Full Name
Elongation factor 1-delta
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
NCBI Official Symbol
EEF1D
NCBI Official Synonym Symbols
EF1D; EF-1D; FP1047
NCBI Protein Information
elongation factor 1-delta; EF-1-delta; antigen NY-CO-4; guanine nucleotide exchange protein
UniProt Protein Name
Elongation factor 1-delta
Protein Family
UniProt Gene Name
EEF1D
UniProt Synonym Gene Names
EF1D; EF-1-delta
UniProt Entry Name
EF1D_HUMAN

NCBI Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]

Uniprot Description

EEF1D: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: eukaryotic translation elongation factor 1 complex; endoplasmic reticulum; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: signal transducer activity; protein binding; transcription activator binding; DNA binding; translation factor activity, nucleic acid binding; heat shock protein binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; translation; transcription, DNA-dependent; mRNA transcription; gene expression; signal transduction

Research Articles on EEF1D

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Product Notes

The EEF1D eef1d (Catalog #AAA4759333) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EEF1D Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EEF1D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:1000-1:5000 IHC-P: 1:50-1:300. Researchers should empirically determine the suitability of the EEF1D eef1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEF1D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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