Rabbit EEF1D Polyclonal Antibody | anti-EEF1D antibody

EEF1D antibody

Cat. Num. AAA9413309

• Gene Names: EEF1D; EF1D; EF-1D; FP1047
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: EEF1D; Polyclonal Antibody; EEF1D antibody; EF1D; EF-1D; FP1047; anti-EEF1D antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total EEF1D antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 647

• Applicable Applications for anti-EEF1D antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human EEF1D.
• Target Name: EEF1D
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using EEF1D antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using EEF1D antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-EEF1D antibody

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.

Product Categories/Family for anti-EEF1D antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 304555583
• NCBI GeneID: 1936
• NCBI Accession #: NP_001123525.2
• NCBI GenBank Nucleotide #: NM_001130053.2
• UniProt Accession #: P29692; Q4VBZ6; Q969J1; Q96I38; B4DDU4; D3DWK3; E9PBQ9
• Molecular Weight: 29,071 Da
• NCBI Official Full Name: elongation factor 1-delta isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• NCBI Official Symbol: EEF1D
• NCBI Official Synonym Symbols: EF1D; EF-1D; FP1047
• NCBI Protein Information: elongation factor 1-delta
• UniProt Protein Name: Elongation factor 1-delta
• Protein Family: Elongation factor
• UniProt Gene Name: EEF1D
• UniProt Synonym Gene Names: EF1D; EF-1-delta
• UniProt Entry Name: EF1D_HUMAN

NCBI Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]

Uniprot Description

EEF1D: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Translation elongation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; eukaryotic translation elongation factor 1 complex; nucleolus; nucleus

Molecular Function: DNA binding; heat shock protein binding; protein binding; signal transducer activity; transcription activator binding; translation elongation factor activity; translation factor activity, nucleic acid binding

Biological Process: cellular protein metabolic process; gene expression; mRNA transcription; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; signal transduction; transcription, DNA-dependent; translation; translational elongation