Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human cervical carcinoma stained with SOX2 Recombinant Rabbit Monoclonal Antibody (SOX2/3811R).)
Rabbit anti-Human, Mouse SOX2 Antibody | anti-SOX2 antibody
SOX2 (Embryonic Stem Cell Marker)
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
SOX2; SOX2 (Embryonic Stem Cell Marker); ANOP3; Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); SOX-2; SRY (sex determining region Y) box 2; SRY related HMG box 2; Transcription factor SOX-2; ysb; anti-SOX2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Isotype
IgG
Clone Number
SOX2/3811R
Specificity
SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
317
Applicable Applications for anti-SOX2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA)
WB: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
WB: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
MCF-7 cells. Lung or Cervical carcinoma.
Immunogen
Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)
Cellular Localization
Nuclear
Hu-chromosome Location
3q26.33
Antibody Type
Recombinant Rabbit Monoclonal Antibody
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human cervical carcinoma stained with SOX2 Recombinant Rabbit Monoclonal Antibody (SOX2/3811R).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human cervical carcinoma stained with SOX2 Recombinant Rabbit Monoclonal Antibody (SOX2/3811R).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human cervical carcinoma stained with SOX2 Recombinant Rabbit Monoclonal Antibody (SOX2/3811R).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human cervical carcinoma stained with SOX2 Recombinant Rabbit Monoclonal Antibody (SOX2/3811R).)
References
Laudet, V., Stehelin, D. and Clevers, H. 1993. Ancestry and diversity of the HMG box superfamily. Nucleic Acids Res. 21: 2493-2501
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34kDa
NCBI Official Full Name
transcription factor SOX-2
NCBI Official Synonym Full Names
SRY-box transcription factor 2
NCBI Official Symbol
SOX2
NCBI Official Synonym Symbols
ANOP3; MCOPS3
NCBI Protein Information
transcription factor SOX-2
UniProt Protein Name
Transcription factor SOX-2
UniProt Gene Name
SOX2
UniProt Entry Name
SOX2_HUMAN
NCBI Description
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
Research Articles on SOX2
Similar Products
Product Notes
The SOX2 sox2 (Catalog #AAA4381258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SOX2 (Embryonic Stem Cell Marker) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SOX2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin. ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA) WB: 1-2ug/ml Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the SOX2 sox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOX2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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