Rabbit EEF1D Polyclonal Antibody | anti-EEF1D antibody

EEF1D Antibody (N-term)

Cat. Num. AAA9202533

• Gene Names: EEF1D; EF1D; EF-1D; FP1047
• Reactivity: Human (Predicted Reactivity: Monkey, Mouse, Rat)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: EEF1D; Polyclonal Antibody; EEF1D Antibody (N-term); Elongation factor 1-delta; EF-1-delta; Antigen NY-CO-4; EF1D; anti-EEF1D antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This EEF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EEF1D.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 262

• Applicable Applications for anti-EEF1D antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of EEF1D antibody (N-term) in Ramos cell line lysates (35ug/lane). EEF1D (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lymph reacted with EEF1D Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EEF1D Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EEF1D antibody

EEF1D is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs.

Product Categories/Family for anti-EEF1D antibody

Signal Transduction

References

Yang,S., BMC Cancer 7, 211 (2007)
Mulner-Lorillon,O., J. Biol. Chem. 269 (31), 20201-20207 (1994)

NCBI and Uniprot Product Information

• NCBI GI #: 304555583
• NCBI GeneID: 1936
• NCBI Accession #: NP_001123525.2; NP_001123527.1; NP_001123528.1; NP_001123529.1; NP_001182132.1; NP_001951.2; NP_115754.3
• NCBI GenBank Nucleotide #: NM_001130053.2
• UniProt Accession #: P29692; Q4VBZ6; Q969J1; Q96I38; B4DDU4; D3DWK3; E9PBQ9
• Molecular Weight: 31122
• NCBI Official Full Name: elongation factor 1-delta isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein)
• NCBI Official Symbol: EEF1D
• NCBI Official Synonym Symbols: EF1D; EF-1D; FP1047
• NCBI Protein Information: elongation factor 1-delta
• UniProt Protein Name: Elongation factor 1-delta
• Protein Family: Elongation factor
• UniProt Gene Name: EEF1D
• UniProt Synonym Gene Names: EF1D; EF-1-delta
• UniProt Entry Name: EF1D_HUMAN

NCBI Description

This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]

Uniprot Description

EEF1D: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. Belongs to the EF-1-beta/EF-1-delta family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: eukaryotic translation elongation factor 1 complex; endoplasmic reticulum; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein binding; signal transducer activity; transcription activator binding; DNA binding; translation factor activity, nucleic acid binding; heat shock protein binding; translation elongation factor activity

Biological Process: translational elongation; cellular protein metabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of transcription, DNA-dependent; transcription, DNA-dependent; translation; mRNA transcription; gene expression; signal transduction