Western Blot (WB)
((1ug/ml) staining of Mouse fetal Lung lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat E2F3 Polyclonal Antibody | anti-E2f3 antibody
Goat anti-E2F3 (mouse) Antibody
Gene Names
E2f3; E2F3b; E2f3a
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
E2F3; Polyclonal Antibody; Goat anti-E2F3 (mouse) Antibody; E2f3; E2F transcription factor 3; E2F3b; E2f3a; mKIAA0075; OTTMUSP00000000663; E2F transcription factor 3 antibody; E2f3a antibody; E2F3b antibody; mKIAA0075 antibody; OTTMUSP00000000663 antibody; E2f3 antibody; E2F3 (mouse); anti-E2f3 antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse, Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DLEKLPLVEDFMCS
Sequence Length
457
Applicable Applications for anti-E2f3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 60kDa and 48kDa band observed in Mouse fetal Lung lysates (calculated MW of 48.8kDa according to NP_034223.1). The 60kDa band was also observed in nuclear lysates of cell line NIH3T3 and it corresponds with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Western Blot: Approx 60kDa and 48kDa band observed in Mouse fetal Lung lysates (calculated MW of 48.8kDa according to NP_034223.1). The 60kDa band was also observed in nuclear lysates of cell line NIH3T3 and it corresponds with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence DLEKLPLVEDFMCS, from the C Terminus of the protein sequence according to NP_034223.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Mouse fetal Lung lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Mouse fetal Lung lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,757 Da
NCBI Official Full Name
transcription factor E2F3 isoform E2f3a
NCBI Official Synonym Full Names
E2F transcription factor 3
NCBI Official Symbol
E2f3
NCBI Official Synonym Symbols
E2F3b; E2f3a
NCBI Protein Information
transcription factor E2F3
UniProt Protein Name
Transcription factor E2F3
Protein Family
UniProt Gene Name
E2f3
UniProt Synonym Gene Names
E2F-3
UniProt Entry Name
E2F3_MOUSE
Uniprot Description
E2F3: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Belongs to the E2F/DP family.
Protein type: DNA-binding; Transcription factor; Cell cycle regulation
Cellular Component: cytoplasm; nucleoplasm; nucleus; transcription factor complex
Molecular Function: DNA binding; protein binding; transcription factor activity
Biological Process: cell cycle; positive regulation of cell proliferation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on E2f3
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Product Notes
The E2f3 e2f3 (Catalog #AAA423225) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-E2F3 (mouse) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's E2F3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 60kDa and 48kDa band observed in Mouse fetal Lung lysates (calculated MW of 48.8kDa according to NP_034223.1). The 60kDa band was also observed in nuclear lysates of cell line NIH3T3 and it corresponds with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the E2f3 e2f3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DLEKLPLVED FMCS. It is sometimes possible for the material contained within the vial of "E2F3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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