Western Blot (WB)
((1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat MST2/STK3 Polyclonal Antibody | anti-STK3 antibody
Goat anti-MST2/STK3 Antibody
Western Blot: Approx 65kDa band observed in lysates of cell lines A431 and NIH3T3 (calculated MW of 56.3kDa according to Human NP_006272.2 and 56.8kDa according to Mouse NP_062609.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.
Protein type: EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Autophagy; Kinase, protein; STE group; STE20 family; MST subfamily
Chromosomal Location of Human Ortholog: 8q22.2
Cellular Component: cytoplasm; cytosol; nucleus; protein complex
Molecular Function: ATP binding; magnesium ion binding; protein binding; protein dimerization activity; protein kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: apoptosis; central nervous system development; negative regulation of cell proliferation; negative regulation of organ growth; neural tube formation; positive regulation of apoptosis; positive regulation of fat cell differentiation; positive regulation of JNK cascade; positive regulation of protein binding; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor activity; protein amino acid phosphorylation; protein stabilization; signal transduction
Research Articles on STK3
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Product Notes
The STK3 stk3 (Catalog #AAA422670) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MST2/STK3 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MST2/STK3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 65kDa band observed in lysates of cell lines A431 and NIH3T3 (calculated MW of 56.3kDa according to Human NP_006272.2 and 56.8kDa according to Mouse NP_062609.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the STK3 stk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DFKNKSHENC NQN. It is sometimes possible for the material contained within the vial of "MST2/STK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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