Rabbit anti-Human DSG2 Polyclonal Antibody | anti-DSG2 antibody

DSG2, CT (DSG2, CDHF5, Desmoglein-2, Cadherin family member 5, HDGC) (Azide free) (HRP)

Cat. Num. AAA6293580

• Gene Names: DSG2; HDGC; CDHF5; ARVC10; ARVD10; CMD1BB
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DSG2; Polyclonal Antibody; CT (DSG2; CDHF5; Desmoglein-2; Cadherin family member 5; HDGC) (Azide free) (HRP); HDGC; anti-DSG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DSG2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DSG2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 829-857 in the C-terminal region of human DSG2, UniProt Accession #Q14126.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS643332 in 293 cell line lysates (35ug/lane).This demonstrates the DSG2 antibody detected the DSG2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS643332 in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-DSG2 antibody

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10.

Product Categories/Family for anti-DSG2 antibody

Antibodies; Cadherin

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Fressart, V., et al. Europace 12(6):861-868(2010). 3. Schlegel, N., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 298 (5), G774-G783 (2010). 4. Cox, M.G., et al. Circ Arrhythm Electrophysiol 3(2):126-133(2010). 5. Buxens, A., et al. Scand J Med Sci Sports (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 116534898
• NCBI GeneID: 1829
• NCBI Accession #: NP_001934.2
• NCBI GenBank Nucleotide #: NM_001943.3
• UniProt Accession #: Q14126; Q4KKU6
• Molecular Weight: 122,294 Da
• NCBI Official Full Name: desmoglein-2 preproprotein
• NCBI Official Synonym Full Names: desmoglein 2
• NCBI Official Symbol: DSG2
• NCBI Official Synonym Symbols: HDGC; CDHF5; ARVC10; ARVD10; CMD1BB
• NCBI Protein Information: desmoglein-2; HDGC; cadherin family member 5
• UniProt Protein Name: Desmoglein-2
• Protein Family: Desmoglein
• UniProt Gene Name: DSG2
• UniProt Synonym Gene Names: CDHF5
• UniProt Entry Name: DSG2_HUMAN

NCBI Description

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. This gene product is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq, Jul 2008]

Uniprot Description

DSG2: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10); also known as arrhythmogenic right ventricular cardiomyopathy 10 (ARVC10). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB). A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Membrane protein, integral; Cell adhesion; Calcium-binding

Chromosomal Location of Human Ortholog: 18q12.1

Cellular Component: desmosome; cell surface; apical plasma membrane; plasma membrane; integral to membrane; intercellular junction; lateral plasma membrane

Molecular Function: calcium ion binding

Biological Process: maternal process involved in pregnancy; apoptosis; homophilic cell adhesion; cell adhesion; response to progesterone stimulus; cell structure disassembly during apoptosis

Disease: Cardiomyopathy, Dilated, 1bb; Arrhythmogenic Right Ventricular Dysplasia, Familial, 10