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Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD57 (TB01) FITC.)

Mouse anti-Human CD57 Monoclonal Antibody | anti-CD57 antibody

Anti-Hu CD57 FITC

Gene Names
B3GAT1; NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD57; Monoclonal Antibody; Anti-Hu CD57 FITC; beta-1; 3-glucuronyltransferase 1; HNK1; LEU7; 3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc; anti-CD57 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM
Clone Number
TB01
Specificity
The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.
Purity/Purification
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide
Sequence Length
334
Applicable Applications for anti-CD57 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
A pool of neuroblastoma cell lines
Conjugation
FITC
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD57 (TB01) FITC.)

Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD57 (TB01) FITC.)
Related Product Information for anti-CD57 antibody
CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and chondroitin sulphate proteoglycans in the nervous system. It serves as a NK cell marker and it is expressed on well differentiated prostate cancers and uveal and cutaneous melanoma. CD57+ T cells are implicated as suppressors of T-cell responses.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform 1
NCBI Official Synonym Full Names
beta-1,3-glucuronyltransferase 1
NCBI Official Symbol
B3GAT1
NCBI Official Synonym Symbols
NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
NCBI Protein Information
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
UniProt Protein Name
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
UniProt Gene Name
B3GAT1
UniProt Synonym Gene Names
GlcUAT-P

NCBI Description

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Research Articles on CD57

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Product Notes

The CD57 b3gat1 (Catalog #AAA1801574) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD57 FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD57 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD57 b3gat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD57, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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