Western Blot (WB)
(Western Blot (WB) analysis of SUPT4H1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)
Rabbit SUPT4H1 Polyclonal Antibody | anti-SUPT4H1 antibody
SUPT4H1 Polyclonal Antibody
Gene Names
SUPT4H1; SPT4; SPT4H; SUPT4H; Supt4a
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SUPT4H1; Polyclonal Antibody; SUPT4H1 Polyclonal Antibody; Transcription elongation factor SPT4; hSPT4; DRB sensitivity-inducing factor 14 kDa subunit; DSIF p14; DRB sensitivity-inducing factor small subunit; DSIF small subunit; SPT4H; SUPT4H; anti-SUPT4H1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SUPT4H1 polyclonal antibody detects endogenous levels of SUPT4H1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
117
Applicable Applications for anti-SUPT4H1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human SUPT4H1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of SUPT4H1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of SUPT4H1 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)
Related Product Information for anti-SUPT4H1 antibody
SPT4 (also designated Suppressor of Ty4 and p14) and SPT5 (also designated DSIF p160) are highly conserved proteins from yeast to humans. Nuclear SPT4 and SPT5 are involved in both DRB (5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the activation of transcriptional elongation by the HIV-1 protein Tat. SPT4 binds SPT5 to form the DSIF (DRB-sensitivity-inducing factor) complex, which binds RNA polymerase II and directly regulates elongation. However, SPT5 protein in mitotic HeLa cells migrates more slowly on SDS-PAGE than does SPT5 isolated from interphase cells, as a result of enhanced SPT5 phosphorylation. The C-terminal CTR1 domain of SPT5 is the substrate for P-TEFb phosphorylation, which is critical for SPT5 function as a regulator of transcriptional elongation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 13kDa
NCBI Official Full Name
SUPT4H1, partial
NCBI Official Synonym Full Names
SPT4 homolog, DSIF elongation factor subunit
NCBI Official Symbol
SUPT4H1
NCBI Official Synonym Symbols
SPT4; SPT4H; SUPT4H; Supt4a
NCBI Protein Information
transcription elongation factor SPT4
UniProt Protein Name
Transcription elongation factor SPT4
UniProt Gene Name
SUPT4H1
UniProt Synonym Gene Names
SPT4H; SUPT4H; hSPT4; DSIF p14; DSIF small subunit
UniProt Entry Name
SPT4H_HUMAN
NCBI Description
This gene encodes the small subunit of DRB (5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
Research Articles on SUPT4H1
Similar Products
Product Notes
The SUPT4H1 supt4h1 (Catalog #AAA3006818) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SUPT4H1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SUPT4H1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the SUPT4H1 supt4h1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUPT4H1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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