TIGAR recombinant protein

TIGAR, 1-270aa, Human, His tag, E Coli

Cat. Num. AAA203860

• Gene Names: TIGAR; FR2BP; C12orf5
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TIGAR; 1-270aa; Human; His tag; E Coli; TP53-induced glycolysis and apoptosis regulator; TIGAR recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR
• Sequence Length: 270

• Applicable Applications for TIGAR recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TIGAR recombinant protein

TIGAR, also known as TP53-induced glycolysis and apoptosis regulator, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. These facts indicate that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses. Recombinant human TIGAR protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TIGAR recombinant protein

Apoptosis

References

Bensaad K., et al. (2009) EMBO J. 28 (19):3015-26.; Hasegawa H., et al. (2009) Leukemia. 23 (11):2090-101.

NCBI and Uniprot Product Information

• NCBI GI #: 9966849
• NCBI GeneID: 57103
• NCBI Accession #: NP_065108
• NCBI GenBank Nucleotide #: NM_020375.2
• UniProt Accession #: Q9NQ88; B2R840
• Molecular Weight: 32.6 kDa (294aa), confirmed by MALDI-TOF
• NCBI Official Full Name: fructose-2,6-bisphosphatase TIGAR
• NCBI Official Synonym Full Names: TP53 induced glycolysis regulatory phosphatase
• NCBI Official Symbol: TIGAR
• NCBI Official Synonym Symbols: FR2BP; C12orf5
• NCBI Protein Information: fructose-2,6-bisphosphatase TIGAR
• UniProt Protein Name: Fructose-2,6-bisphosphatase TIGAR
• Protein Family: Fructose-2,6-bisphosphatase
• UniProt Gene Name: TIGAR

NCBI Description

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]

Uniprot Description

Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (PubMed:19015259). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:16839880, PubMed:22887998). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:16839880, PubMed:19713938, PubMed:23726973, PubMed:22887998, PubMed:23817040). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (PubMed:23185017). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (). Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed:23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner (PubMed:22887998). Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (PubMed:25928429). Involved in intestinal tumor progression (PubMed:23726973).