Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit | HIF2a elisa kit

Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit

Cat. Num. AAA2705121

• Gene Names: Epas1; HLF; Hif2a; HIF2 alpha; HIF-2 alpha
• Reactivity: Rat
• Synonyms: Hypoxia Inducible Factor 2 Alpha (HIF2a); Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit; EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; HIF2a elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 2 Alpha (HIF2a).; No significant cross-reactivity or interference between Hypoxia Inducible Factor 2 Alpha (HIF2a) and analogues was observed.
• Sequence Length: 874

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 78-5000pg/mL
• Sensitivity: < 29pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 2 Alpha (HIF2a) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Inducible Factor 2 Alpha (HIF2a) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIF2a elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 2 Alpha (HIF2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 2 Alpha (HIF2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8953577
• NCBI GeneID: 29452
• UniProt Accession #: Q9JHS1
• Molecular Weight: 96,718 Da
• NCBI Official Full Name: hypoxia inducible factor 2 alpha
• NCBI Official Synonym Full Names: endothelial PAS domain protein 1
• NCBI Official Symbol: Epas1
• NCBI Official Synonym Symbols: HLF; Hif2a; HIF2 alpha; HIF-2 alpha
• NCBI Protein Information: endothelial PAS domain-containing protein 1
• UniProt Protein Name: Endothelial PAS domain-containing protein 1
• UniProt Gene Name: Epas1
• UniProt Synonym Gene Names: Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha

NCBI Description

activates luciferase activity after transfection [RGD, Feb 2006]

Uniprot Description

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD ().