SDS-Page
PTH active protein
PTH, 32-115aa, Human, E Coli (Bioactivity Validated)
Gene Names
PTH; PTH1
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
PTH; 32-115aa; Human; E Coli (Bioactivity Validated); PTH1; PTH active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) containing 2 mM EDTA
Concentration
1 mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence
MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ
Sequence Length
115
Applicable Applications for PTH active protein
SDS-PAGE
Antigen Species
Human
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 for this effect is less than or equal to 50 ng/ml.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PTH active protein
Human parathyroid hormone (hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone (amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques
Product Categories/Family for PTH active protein
References
Hodsman AB. et al. (2003) J. Clin. Endocrinol. Metab. 88 (11), 5212-20.; Wingender E., et al. (1989) J. Biol. Chem. 264 (8),4367-73.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9.55 kDa (85 aa), confirmed by MALDI-TOF
NCBI Official Full Name
parathyroid hormone isoform 1 preproprotein
NCBI Official Synonym Full Names
parathyroid hormone
NCBI Official Symbol
PTH
NCBI Official Synonym Symbols
PTH1
NCBI Protein Information
parathyroid hormone
UniProt Protein Name
Parathyroid hormone
Protein Family
UniProt Gene Name
PTH
UniProt Synonym Gene Names
PTH
NCBI Description
This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Uniprot Description
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Research Articles on PTH
Similar Products
Product Notes
The PTH pth (Catalog #AAA206164) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PTH can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PTH pth for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ. It is sometimes possible for the material contained within the vial of "PTH, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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