Human Syndecan-3/SDC3 ELISA Kit | SDC3 elisa kit

Human Syndecan-3/SDC3 PicoKine ELISA Kit

Cat. Num. AAA178743

• Gene Names: SDC3; SDCN; SYND3
• Reactivity: Human
• Synonyms: Syndecan-3/SDC3; Human Syndecan-3/SDC3 PicoKine ELISA Kit; N-syndecan; Sdc3; SDC3_HUMAN; SDCN; SYND3; Syndecan neural type; syndecan proteoglycan 3; Syndecan-3; Syndecan 3; O75056; SDC3 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Syndecan-3
• Sequence Length: 442

• Assay Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 20-25
• Immunogen: NSO, A41-V387
• Sample Data: Concentration(pg/ml): |0|156|312|625|1250|2500|5000|10000; O.D.: |0.011|0.030|0.063|0.124|0.282|0.656|1.353|2.398
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |177|695|3112|186|987|4569; Standard Deviation: |5.84|25.02|108.92|6.32|37.50|164.48; CV(%): |3.3|3.6|3.5|3.4|3.8|3.6
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human Syndecan-3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each human Syndecan-3 standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human Syndecan-3/SDC3 PicoKine ELISA Kit standard curve )

Related Product Information for SDC3 elisa kit

For quantitative detection of human Syndecan-3 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma(heparin, EDTA).
Background: Syndecan-3 is a protein that in humans is encoded by the SDC3 gene. The protein encoded by this gene belongs to the syndecan proteoglycan family, which was assigned to human chromosome 1p35.2, and it showed that in the mouse the Synd3 gene maps to chromosome 4 near the Lmyc gene. The transfection of SDC3 resulted in the formation of long filopodia-like structures, microspikes, and varicosities in several cell lines. Besides, the gene plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.
Principle: Human Syndecan-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for Syndecan-3 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A41-V387) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Syndecan-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Syndecan-3 amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 187960059
• NCBI GeneID: 9672
• NCBI Accession #: NP_055469.3
• NCBI GenBank Nucleotide #: NM_014654.3
• UniProt Accession #: O75056; Q5T1Z6; Q5T1Z7; Q96CT3; Q96PR8
• Molecular Weight: 45,497 Da
• NCBI Official Full Name: syndecan-3
• NCBI Official Synonym Full Names: syndecan 3
• NCBI Official Symbol: SDC3
• NCBI Official Synonym Symbols: SDCN; SYND3
• NCBI Protein Information: syndecan-3
• UniProt Protein Name: Syndecan-3
• Protein Family: Syndecan
• UniProt Gene Name: SDC3
• UniProt Synonym Gene Names: KIAA0468; SYND3

NCBI Description

The protein encoded by this gene belongs to the syndecan proteoglycan family. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity. [provided by RefSeq, Oct 2009]

Uniprot Description

syndecan-3: Cell surface proteoglycan that may bear heparan sulfate. May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Belongs to the syndecan proteoglycan family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p35.2

Cellular Component: cell surface; Golgi lumen; lysosomal lumen; membrane; plasma membrane

Biological Process: cell migration; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; retinoid metabolic process

Disease: Obesity