Rabbit anti-Human DOK1 Polyclonal Antibody | anti-TEP1 antibody

DOK1 Antibody

Cat. Num. AAA151146

• Gene Names: TEP1; TP1; TLP1; p240; TROVE1; VAULT2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: DOK1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: DOK1; Polyclonal Antibody; DOK1 Antibody; TP1; TLP1; p240; TROVE1; VAULT2; Docking protein 1; Downstream of tyrosine kinase 1; telomerase-associated protein 1; anti-TEP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: DOK1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 2627

• Applicable Applications for anti-TEP1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: DOK1 antibody can be used for detection of DOK1 expression by Western blot at 1 mug/mL. A 62 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: DOK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1.
• Buffer: DOK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DOK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of DOK1 in K562 cells with DOK1 antibody at 10 μg/ml.)

Related Product Information for anti-TEP1 antibody

DOK1 Antibody: Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.

NCBI and Uniprot Product Information

• NCBI GI #: 1848277
• NCBI GeneID: 7011
• UniProt Accession #: Q99704; A0AUV9; Q99973
• Molecular Weight: 62 kDa
• NCBI Official Full Name: telomerase-associated protein TP-1
• NCBI Official Synonym Full Names: telomerase-associated protein 1
• NCBI Official Symbol: TEP1
• NCBI Official Synonym Symbols: TP1; TLP1; p240; TROVE1; VAULT2
• NCBI Protein Information: telomerase protein component 1; telomerase protein 1; p80 telomerase homolog; TROVE domain family, member 1
• UniProt Protein Name: Telomerase protein component 1
• Protein Family: Telomerase protein component
• UniProt Gene Name: TEP1
• UniProt Synonym Gene Names: TLP1; TP1; Telomerase protein 1
• UniProt Entry Name: TEP1_HUMAN

NCBI Description

This gene product is a component of the ribonucleoprotein complex responsible for telomerase activity which catalyzes the addition of new telomeres on the chromosome ends. The telomerase-associated proteins are conserved from ciliates to humans. [provided by RefSeq, Jul 2008]

Uniprot Description

DOK1: a docking protein that interacts with receptor tyrosine kinases. It is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; receptor signaling protein activity; insulin receptor binding

Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway