Rabbit anti-Human Dok2, p56 Polyclonal Antibody | anti-DOK2 antibody

Dok2, p56, phosphorylated (Tyr351) (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP, rasGAP-associated docking protein)

Cat. Num. AAA615420

• Gene Names: DOK2; p56DOK; p56dok-2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Protein A and peptide affinity chromatography
• Synonyms: Dok2; p56; Polyclonal Antibody; phosphorylated (Tyr351) (Docking Protein; Downstream of Tyrosine Kinase; Dok-R; p56Dok2; FRIP; rasGAP-associated docking protein); Anti -Dok2; anti-DOK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of p56Dok-2 only when phosphorylated at tyrosine 351. Does not crossreact with other tyrosine phosphorylated p62Dok family members.
• Purity/Purification: Affinity Purified; Protein A and peptide affinity chromatography
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol

• Applicable Applications for anti-DOK2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding tyrosine 351 of human p56Dok-2
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Western Blot (WB)

(Western Blot analysis of cell extracts from Jurkat cells over expressing p56Dok-2 proteins untreated or stimulated with anti-CD2 antibody using MBS615420 or D8080-02 (lower).)

Related Product Information for anti-DOK2 antibody

Docking proteins are substrates of tyrosine kinases and function in the recruitment and assembly of specific signal transduction molecules. There are five members in p62dok family including p62Dok (Dok-1), p56Dok-2 (Dok-2, or DoK-R), Dok-3, Dok-4 and Dok-5 (1-3), which are characterized by the presence of an amino-terminal PH domain, a central PTB domain and numerous potential sites of tyrosine phosphorylation. Tyrosine phosphorylation of p56Dok-2 occurs upon stimulation of cells with a variety of stimuli or in cells transformed by oncogenic tyrosine kinases such as v-Src and Bcr-Abl (3-5). Very little is known about p56Dok-2 function and the role of phosphorylation in the modulation of these functions. Based on the presence of several signaling domains (PH, PTB domain, tyrosine residue and proline-rich regions), it has been proposed that they act as docking proteins that link RTKs to signal transduction pathways. p56Dok-2 has been proposed to be a negative regulator of cytokine-induced proliferation in T cells (5). It was demonstrated that phosphorylated Tyr351 mediates an association with the SH2 domain of Nck (4).

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok. Human DOK1 is a 481 amino acid protein.

Product Categories/Family for anti-DOK2 antibody

Antibodies; Abs to Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 41406050
• NCBI GeneID: 9046
• NCBI Accession #: NP_003965.2
• NCBI GenBank Nucleotide #: NM_003974.2
• UniProt Accession #: O60496; Q8N5A4
• Molecular Weight: 45,379 Da
• NCBI Official Full Name: docking protein 2
• NCBI Official Synonym Full Names: docking protein 2, 56kDa
• NCBI Official Symbol: DOK2
• NCBI Official Synonym Symbols: p56DOK; p56dok-2
• NCBI Protein Information: docking protein 2; p56(dok-2); OTTHUMP00000161710; OTTHUMP00000224923; downstream of tyrosine kinase 2
• UniProt Protein Name: Docking protein 2
• Protein Family: Docking protein
• UniProt Gene Name: DOK2
• UniProt Entry Name: DOK2_HUMAN

NCBI Description

The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq]

Uniprot Description

DOK2: a member of the p62dok family of proteins that interact with receptor tyrosine kinases and mediate particular biological responses. Constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/Abl), a chimeric protein whose presence is associated with CML. Contains a pleckstrin homology domain at the amino terminus, 13 potential tyrosine phosphorylation sites, and six PXXP SH3 recognition motifs. Binds p120 (RasGAP) from CML cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: cytosol

Molecular Function: receptor signaling protein activity; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; signal transduction; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway