Rabbit anti-Human Dok, p62 Polyclonal Antibody | anti-DOK6 antibody

Dok, p62, CT (Dok-1, Docking Protein, Downstream of Tyrosine Kinase, FRIP)

Cat. Num. AAA626737

• Gene Names: DOK6; DOK5L; HsT3226
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified; Purified IgG
• Synonyms: Dok; p62; Polyclonal Antibody; CT (Dok-1; Docking Protein; Downstream of Tyrosine Kinase; FRIP); Anti -Dok; anti-DOK6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A 62kD band should be detected.
• Purity/Purification: Purified; Purified IgG
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-DOK6 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000 to 1:2000. A 62kD band should be detected.
• Immunogen: Raised against a peptide corresponding to amino acids 425-439 of human p62dok (1).
• Positive Control: Whole cell lysate from Jurkat cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MBS626737.)

Testing Data

Related Product Information for anti-DOK6 antibody

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok. Human DOK1 is a 481 amino acid protein.

Docking proteins are substrates of tyrosine kinases and function in the recruitment and assembly of specific signal transduction molecules. Here we found that p62dok family members act as substrates for the c-Ret receptor tyrosine kinase. In addition to dok-1, dok-2, and dok-3, two new family members, dok-4 and dok-5 have been identified that can directly associate with Y1062 of c-Ret. Dok-4 and dok-5 constitute a subgroup of dok family members that is coexpressed with c-Ret in various neuronal tissues. Activated c-Ret promotes neurite outgrowth of PC12 cells; for this activity, Y1062 in c-Ret is essential. c-Ret/dok fusion proteins, in which Y1062 of c-Ret is deleted and replaced by the sequences of dok-4 or dok-5, induce ligand-dependent axonal outgrowth of PC12 cells, whereas a c-Ret fusion containing dok-2 sequences does not elicit this response. Dok-4 and dok-5 do not associate with rasGAP or Nck, in contrast to p62dok and dok-2. Moreover, dok-4 and dok-5 enhance c-Ret-dependent activation of mitogen-activated protein kinase.

Product Categories/Family for anti-DOK6 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 47575849
• NCBI GeneID: 220164
• NCBI Accession #: NP_689934.2
• NCBI GenBank Nucleotide #: NM_152721.5
• UniProt Accession #: Q6PKX4; Q4V9S3; Q8WUZ8; Q96HI2; Q96LU2; A6NNG3
• Molecular Weight: 38,318 Da
• NCBI Official Full Name: docking protein 6
• NCBI Official Synonym Full Names: docking protein 6
• NCBI Official Symbol: DOK6
• NCBI Official Synonym Symbols: DOK5L; HsT3226
• NCBI Protein Information: docking protein 6; downstream of tyrosine kinase 6
• UniProt Protein Name: Docking protein 6
• Protein Family: Docking protein
• UniProt Gene Name: DOK6
• UniProt Synonym Gene Names: DOK5L
• UniProt Entry Name: DOK6_HUMAN

NCBI Description

DOK6 is a member of the DOK (see DOK1; MIM 602919) family of intracellular adaptors that play a role in the RET (MIM 164761) signaling cascade (Crowder et al., 2004 [PubMed 15286081]).[supplied by OMIM, Mar 2008]

Uniprot Description

DOK6: ocking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. Interacts via its PTB domain with phosphorylated Ret, promoting neurite growth. May have a role in brain development and/or maintenance. Highly expressed in fetal and adult brain. Highly expressed in the cerebellum. Weak expression in kidney, spinal cord and testis.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 18q22.2

Molecular Function: protein binding; insulin receptor binding