Rabbit anti-Human Dok, p62 Polyclonal Antibody | anti-DOK6 antibody

Dok, p62, CT (Dok-1, Docking Protein, Downstream of Tyrosine Kinase, FRIP)

Cat. Num. AAA630304

• Gene Names: DOK6; DOK5L; HsT3226
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Synonyms: Dok; p62; Polyclonal Antibody; CT (Dok-1; Docking Protein; Downstream of Tyrosine Kinase; FRIP); Anti -Dok; anti-DOK6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects overxpressed levels of p62Dok proteins. It does not crossreact with any other related proteins.
• Purity/Purification: Affinity Purified; Purified by protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

• Applicable Applications for anti-DOK6 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding tyrosine 398 of human p62Dok.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer

Western Blot (WB)

(Western blot analysis of cell extracts from CHO cells transfected with Wild-type (WT) and mutant (Y362F) p62Dok-expression constructs, using p62Dok Antibody.)

Related Product Information for anti-DOK6 antibody

Docking proteins are substrates of tyrosine kinases and function in the recruitment and assembly of specific signal transduction molecules. There are five members in p62dok family including p62Dok (Dok-1), p56Dok-2 (Dok-2, or DoK-R), Dok-3, Dok-4 and Dok-5 (1-3), which are characterized by the presence of an amino-terminal PH domain, a central PTB domain and numerous potential sites of tyrosine phosphorylation. Tyrosine phosphorylation of p56Dok-2 occurs upon stimulation of cells with a variety of stimuli or in cells transformed by oncogenic tyrosine kinases such as v-Src and Bcr-Abl (3-5). Very little is known about p56Dok-2 function and the role of phosphorylation in the modulation of these functions. Based on the presence of several signaling domains (PH, PTB domain, tyrosine residue and proline-rich regions), it has been proposed that they act as docking proteins that link RTKs to signal transduction pathways. p56Dok-2 has been proposed to be a negative regulator of cytokine-induced proliferation in T cells (5). It was demonstrated that phosphorylated Tyr351 mediates an association with the SH2 domain of Nck (4).

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok. Human DOK1 is a 481 amino acid protein.

Product Categories/Family for anti-DOK6 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 47575849
• NCBI GeneID: 220164
• NCBI Accession #: NP_689934.2
• NCBI GenBank Nucleotide #: NM_152721.5
• UniProt Accession #: Q6PKX4; Q4V9S3; Q8WUZ8; Q96HI2; Q96LU2; A6NNG3
• Molecular Weight: 38,318 Da
• NCBI Official Full Name: docking protein 6
• NCBI Official Synonym Full Names: docking protein 6
• NCBI Official Symbol: DOK6
• NCBI Official Synonym Symbols: DOK5L; HsT3226
• NCBI Protein Information: docking protein 6; downstream of tyrosine kinase 6
• UniProt Protein Name: Docking protein 6
• Protein Family: Docking protein
• UniProt Gene Name: DOK6
• UniProt Synonym Gene Names: DOK5L
• UniProt Entry Name: DOK6_HUMAN

NCBI Description

DOK6 is a member of the DOK (see DOK1; MIM 602919) family of intracellular adaptors that play a role in the RET (MIM 164761) signaling cascade (Crowder et al., 2004 [PubMed 15286081]).[supplied by OMIM, Mar 2008]

Uniprot Description

DOK6: ocking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. Interacts via its PTB domain with phosphorylated Ret, promoting neurite growth. May have a role in brain development and/or maintenance. Highly expressed in fetal and adult brain. Highly expressed in the cerebellum. Weak expression in kidney, spinal cord and testis.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 18q22.2

Molecular Function: protein binding; insulin receptor binding