Human glycoprotein VI (platelet) ELISA Kit | GP6 elisa kit

Human Platelet glycoprotein VI, GP6 ELISA Kit

Cat. Num. AAA915183

• Gene Names: GP6; GPIV; GPVI; BDPLT11
• Reactivity: Human
• Synonyms: glycoprotein VI (platelet); Human Platelet glycoprotein VI; GP6 ELISA Kit; Human Platelet glycoprotein VI (GP6) ELISA kit; GPIV; GPVI; MGC138168; platelet collagen receptor; platelet glycoprotein VI; GP6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human GP6. No significant cross-reactivity or interference between human GP6 and analogues was observed.
• Sequence Length: 620

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 46.88 pg/ml-3000 pg/ml
• Sensitivity: < 11.72 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GP6 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GP6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 563580292
• NCBI GeneID: 51206
• NCBI Accession #: NP_001077368.2
• NCBI GenBank Nucleotide #: NM_001083899.2
• UniProt Accession #: Q9HCN6; Q9HCN7; Q9UIF2
• Molecular Weight: 36,866 Da
• NCBI Official Full Name: platelet glycoprotein VI isoform 1
• NCBI Official Synonym Full Names: glycoprotein VI (platelet)
• NCBI Official Symbol: GP6
• NCBI Official Synonym Symbols: GPIV; GPVI; BDPLT11
• NCBI Protein Information: platelet glycoprotein VI; glycoprotein 6; platelet collagen receptor
• UniProt Protein Name: Platelet glycoprotein VI
• Protein Family: Platelet glycoprotein
• UniProt Gene Name: GP6
• UniProt Synonym Gene Names: GPVI
• UniProt Entry Name: GPVI_HUMAN

NCBI Description

This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

GPVI: Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma- chain, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. Defects in GP6 are the cause of bleeding disorder platelet-type 11 (BDPLT11). BDPLT11 is a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: collagen binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: platelet activation; blood coagulation; leukocyte migration; enzyme linked receptor protein signaling pathway

Disease: Bleeding Disorder, Platelet-type, 11