Rabbit anti-Human DGK-theta Polyclonal Antibody | anti-DGK-theta antibody

DGK-theta (S725) Polyclonal Antibody

Cat. Num. AAA3002676

• Gene Names: DGKQ; DAGK; DAGK4; DAGK7
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DGK-theta; Polyclonal Antibody; DGK-theta (S725) Polyclonal Antibody; DGKQ; DAGK4; Diacylglycerol kinase theta; anti-DGK-theta antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. The accumulation of DAG in the nucleus is important for the regulation of cell growth and differentiation.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 942

• Applicable Applications for anti-DGK-theta antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 700-750 of Human DGK-theta.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of DGK-theta (S725) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-DGK-theta antibody

Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. The accumulation of DAG in the nucleus is important for the regulation of cell growth and differentiation. Diacylglycerol kinases (DGKs) convert DAG to phosphatidic acid, thereby terminating diacylglycerol signaling, which results in the reduction of protein kinase C activity and cell cycle progression of T lymphocytes. Diacylglycerol kinases are divided into five subtypes, Type I-Type V. DGK-theta is a Type V DGK, and localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12 and HeLa. Nuclear DGK-theta co-localizes with phosphatidylinositol 4, 5-bisphosphate (PIP(2)). DGK-theta is the isoform responsive to alpha-Thrombin stimulation.

NCBI and Uniprot Product Information

• NCBI GI #: 40806175
• NCBI GeneID: 1609
• NCBI Accession #: NP_001338.2
• NCBI GenBank Nucleotide #: NP_001338.2
• UniProt Accession #: P52824
• Molecular Weight: ~ 101kDa
• NCBI Official Full Name: diacylglycerol kinase theta
• NCBI Official Synonym Full Names: diacylglycerol kinase theta
• NCBI Official Symbol: DGKQ
• NCBI Official Synonym Symbols: DAGK; DAGK4; DAGK7
• NCBI Protein Information: diacylglycerol kinase theta
• UniProt Protein Name: Diacylglycerol kinase theta
• UniProt Gene Name: DGKQ
• UniProt Synonym Gene Names: DAGK4; DAG kinase theta; DGK-theta
• UniProt Entry Name: DGKQ_HUMAN

NCBI Description

The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; EC 2.7.1.107

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; nuclear speck; cytosol; nucleus

Molecular Function: protein binding; phospholipase binding; transcription activator binding; metal ion binding; kinase binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; platelet activation; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; protein kinase C activation; blood coagulation; phosphorylation; response to ATP